The presence of small ruminant lentiviruses in Mexican Pelibuey sheep
Por:
Sanchez, Jose H., Martinez, Humberto A., Garcia, Maria M., Garrido, German, Gomez, Luis, Aguilar, Jose A., de Andres, Damian F., Reina, Ramses, Ramirez, Hugo
Publicada:
1 nov 2016
Resumen:
The transmission frequency of small ruminant lentiviruses (SRLVs)
through the placenta is controversial and may be associated with breed
susceptibility. In Mexico, SRLV infections in sheep have been poorly
studied. This work explores the presence of antibodies and proviral DNA
in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs;
three commercial kits and two on the basis of synthetic peptides) and
polymerase chain reaction (PCR; amplifying the long terminal repeat and
gag segments) were performed to diagnose SRLV infection in 25 adult
Pelibuey ewes with an average age of 2.5 years and 32 fetuses with
gestational ages ranging from 40 to 90 days without clinical signs of
SRLV. Two of the three commercial ELISAs and the synthetic peptide-based
ones were positive for SRLV antibody detection in 28% and 24% of the
ewes, respectively, whereas none of the fetuses were positive by any of
the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses
were positive. Characteristic SRLV lesions were not found in the fetal
and/or ewe tissues, including those with positive PCR results. These
findings demonstrate the susceptibility of Pelibuey sheep to SRLV
infection and the low transmission frequency through the placenta. (C)
2016 Elsevier Inc. All rights reserved.
Filiaciones:
Sanchez, Jose H.:
Univ Nacl Autonoma Mexico, FESC, Lab Virol Genet & Biol Mol, Campo 4, Mexico City, Estado De Mexic, Mexico
Martinez, Humberto A.:
Univ Nacl Autonoma Mexico, FESC, Lab Virol Genet & Biol Mol, Campo 4, Mexico City, Estado De Mexic, Mexico
Garcia, Maria M.:
UMAE Hosp Pediat, Ctr Med Nacl Siglo 21, Inst Mexicano Seguro Social, Lab Inmunovirol,Unidad Invest Med Inmunol, Ciudad De Mexico, Mexico
Garrido, German:
Univ Nacl Autonoma Mexico, FESC, Lab Apoyo Histol & Biol, Campo 4, Mexico City, Estado De Mexic, Mexico
Gomez, Luis:
INIFAP, Lab Virol CENID Microbiol Anim, Ciudad De Mexico, Mexico
Aguilar, Jose A.:
UMAE Hosp Pediat, Ctr Med Nacl Siglo 21, Inst Mexicano Seguro Social, Lab Inmunovirol,Unidad Invest Med Inmunol, Ciudad De Mexico, Mexico
de Andres, Damian F.:
UPNA CSIC Gobierno Navarra, Inst Agrobiotecnol, Navarra, Spain
Reina, Ramses:
UPNA CSIC Gobierno Navarra, Inst Agrobiotecnol, Navarra, Spain
Ramirez, Hugo:
Univ Nacl Autonoma Mexico, FESC, Lab Virol Genet & Biol Mol, Campo 4, Mexico City, Estado De Mexic, Mexico
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