Transcriptomic analysis of the process of biofilm formation in Rhizobium etli CFN42
Por:
Reyes-Perez, Agustin, del Carmen Vargas, Maria, Hernandez, Magdalena, Aguirre-von-Wobeser, Eneas, Perez-Rueda, Ernesto, Encarnacion, Sergio
Publicada:
1 nov 2016
Resumen:
Organisms belonging to the genus Rhizobium colonize leguminous plant
roots and establish a mutually beneficial symbiosis. Biofilms are
structured ecosystems in which microbes are embedded in a matrix of
extracellular polymeric substances, and their development is a multistep
process. The biofilm formation processes of R. etli CFN42 were analyzed
at an early (24-h incubation) and mature stage (72 h), comparing cells
in the biofilm with cells remaining in the planktonic stage. A
genome-wide microarray analysis identified 498 differentially regulated
genes, implying that expression of similar to 8.3 % of the total R.
etli gene content was altered during biofilm formation. In
biofilms-attached cells, genes encoding proteins with diverse functions
were over-expressed including genes involved in membrane synthesis,
transport and chemotaxis, repression of flagellin synthesis, as well as
surface components (particularly exopolysaccharides and
lipopolysaccharides), in combination with the presence of activators or
stimulators of N-acyl-homoserine lactone synthesis This suggests that R.
etli is able to sense surrounding environmental conditions and
accordingly regulate the transition from planktonic and biofilm growth.
In contrast, planktonic cells differentially expressed genes associated
with transport, motility (flagellar and twitching) and inhibition of
exopolysaccharide synthesis. To our knowledge, this is the first report
of nodulation and nitrogen assimilation-related genes being involved in
biofilm formation in R. etli. These results contribute to the
understanding of the physiological changes involved in biofilm formation
by bacteria.
Filiaciones:
Reyes-Perez, Agustin:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Posgrad Ciencias Biol, Apartado Postal 70-153, Cuernavaca 0415, Morelos, Mexico
Univ Autonoma Estado Morelos, Ctr Invest Dinam Celular, Ave Univ 1001, Cuernavaca 62209, Morelos, Mexico
del Carmen Vargas, Maria:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico
Hernandez, Magdalena:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico
Aguirre-von-Wobeser, Eneas:
Inst Ecol AC, Red Estudios Mol Avanzados, Coatepec 351, Xalapa, Veracruz, Mexico
Perez-Rueda, Ernesto:
UNAM, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Ave Univ S-N, Cuernavaca, Morelos, Mexico
Encarnacion, Sergio:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico
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