Active tuberculosis patients have high levels of IgA anti-alphacrystallin and isocitrate lyase proteins
Por:
Talavera-Paulin, M., Garcia-Morales, L., Ruiz-Sanchez, B. P., Caamal-Ley, A. D., Hernandez-Solis, A., Ramirez-Casanova, E., Cicero-Sabido, R., Espitia, C., Helguera-Repetto, C., Gonzalez-y-Merchand, J. A., Flores-Mejia, R., Estrada-Parra, S., Estrada-Garcia, I., Chacon-Salinas, R., Wong-Baeza, I., Serafin-Lopez, J.
Publicada:
1 dic 2016
Resumen:
SETTING: Mexico City, Mexico.
OBJECTIVE: To identify proteins synthetised by Mycobacterium
tuberculosis in hypoxic culture, which resemble more closely a granuloma
environment than aerobic culture, and to determine if they are
recognised by antibodies from patients with active pulmonary
tuberculosis (PTB).
DESIGN: Soluble extracts from M. tuberculosis H37Rv cultured under
aerobic or hypoxic conditions were analysed using two-dimensional
polyacrylamide gel electrophoresis, and proteins over-expressed under
hypoxia were identified by mass spectrometry. The presence of
immunoglobulin (Ig) G, IgA and IgM antibodies against these proteins was
determined in the serum of 42 patients with active PTB and 42 healthy
controls.
RESULTS: We selected three M. tuberculosis H37Rv proteins
(alpha-crystallin protein [Acr, Rv2031c], universal stress protein
Rv2623 and isocitrate lyase [ICL, RV0467]) that were over-expressed
under hypoxia. Titres of anti-Acr and anti-ICL IgA antibodies were
higher in patients than in healthy controls, with an area under the
receiver operating characteristic curve of 0.71 for anti-ICL IgA
antibodies.
CONCLUSION: ICL could be used in combination with other M. tuberculosis
antigens to improve the sensitivity and specificity of current
serological TB diagnostic methods.
Filiaciones:
Talavera-Paulin, M.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
IPN, Escuela Nacl Ciencias Biol, Programa Posgrad Inmunol, Mexico City, DF, Mexico
Garcia-Morales, L.:
IPN, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico
IPN, Escuela Nacl Ciencias Biol, Programa Posgrad Biomed & Biotecnol Mol, Mexico City, DF, Mexico
Ruiz-Sanchez, B. P.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
IPN, Escuela Nacl Ciencias Biol, Programa Posgrad Inmunol, Mexico City, DF, Mexico
Caamal-Ley, A. D.:
Univ Autonoma Yucatan, Ctr Invest Reg Dr Hideyo Noguchi, Merida, Yucatan, Mexico
Hernandez-Solis, A.:
Univ Nacl Autonoma Mexico, Secretaria Salud, Serv Neumol, Hosp Gen Mexico Dr Eduardo Liceaga,Fac Med, Mexico City, DF, Mexico
Ramirez-Casanova, E.:
Univ Nacl Autonoma Mexico, Secretaria Salud, Serv Neumol, Hosp Gen Mexico Dr Eduardo Liceaga,Fac Med, Mexico City, DF, Mexico
Cicero-Sabido, R.:
Univ Nacl Autonoma Mexico, Secretaria Salud, Serv Neumol, Hosp Gen Mexico Dr Eduardo Liceaga,Fac Med, Mexico City, DF, Mexico
Espitia, C.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City, DF, Mexico
Helguera-Repetto, C.:
Isidro Espinosa de los Reyes Inst Nacl Perinatol, Mexico City, DF, Mexico
Gonzalez-y-Merchand, J. A.:
IPN, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico
Flores-Mejia, R.:
IPN, Escuela Super Med, Dept Inmunol, Mexico City, DF, Mexico
Estrada-Parra, S.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
Estrada-Garcia, I.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
Chacon-Salinas, R.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
Wong-Baeza, I.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
Serafin-Lopez, J.:
IPN, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico
|