Genomes of Candidatus Wolbachia bourtzisii wDacA and Candidatus Wolbachia pipientis wDacB from the Cochineal Insect Dactylopius coccus (Hemiptera: Dactylopiidae)
Por:
Ramirez-Puebla, Shamayim T., Ormeno-Orrillo, Ernesto, Vera-Ponce de Leon, Arturo, Lozano, Luis, Sanchez-Flores, Alejandro, Rosenblueth, Monica, Martinez-Romero, Esperanza
Publicada:
1 oct 2016
Resumen:
Dactylopius species, known as cochineal insects, are the source of the
carminic acid dye used worldwide. The presence of two Wolbachia strains
in Dactylopius coccus from Mexico was revealed by PCR amplification of
wsp and sequencing of 16S rRNA genes. A metagenome analysis recovered
the genome sequences of Candidatus Wolbachia bourtzisii wDacA
(supergroup A) and Candidatus Wolbachia pipientis wDacB (supergroup B).
Genome read coverage, as well as 16S rRNA clone sequencing, revealed
that wDacB was more abundant than wDacA. The strains shared similar
predicted metabolic capabilities that are common to Wolbachia, including
riboflavin, ubiquinone, and heme biosynthesis, but lacked other vitamin
and cofactor biosynthesis as well as glycolysis, the oxidative pentose
phosphate pathway, and sugar uptake systems. A complete tricarboxylic
acid cycle and gluconeogenesis were predicted as well as limited amino
acid biosynthesis. Uptake and catabolism of proline were evidenced in
Dactylopius Wolbachia strains. Both strains possessed WO-like phage
regions and type I and type IV secretion systems. Several efflux systems
found suggested the existence of metal toxicity within their host.
Besides already described putative virulence factors like ankyrin domain
proteins, VlrC homologs, and patatin-like proteins, putative novel
virulence factors related to those found in intracellular pathogens like
Legionella and Mycobacterium are highlighted for the first time in
Wolbachia. Candidate genes identified in other Wolbachia that are likely
involved in cytoplasmic incompatibility were found in wDacB but not in
wDacA.
Filiaciones:
Ramirez-Puebla, Shamayim T.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ SN, Cuernavaca 62210, Morelos, Mexico
Ormeno-Orrillo, Ernesto:
Univ Nacl Agr Molina, Lab Ecol Microbiana & Biotecnol Marino Tabusso, Lima 12, Peru
Vera-Ponce de Leon, Arturo:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ SN, Cuernavaca 62210, Morelos, Mexico
Lozano, Luis:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ SN, Cuernavaca 62210, Morelos, Mexico
Sanchez-Flores, Alejandro:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Rosenblueth, Monica:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ SN, Cuernavaca 62210, Morelos, Mexico
Martinez-Romero, Esperanza:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ SN, Cuernavaca 62210, Morelos, Mexico
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