SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques
Por:
De La Mora E., Flores-Hernández E., Jakoncic J., Stojanoff V., Siliqi D., Sánchez-Puig N., Moreno A.
Publicada:
1 oct 2015
Categoría:
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Resumen:
SdsA, a sodium dodecyl sulfate hydrolase, from Pseudomonas aeruginosa was crystallized in three different crystal polymorphs and their three-dimensional structure was determined. The different polymorphs present different crystal packing habits. One of the polymorphs suggests the existence of a tetramer, an oligomeric state not observed previously, while the crystal packing of the remaining two polymorphs obstructs the active site entrance but stabilizes flexible regions of the protein. Nonconventional crystallization methods that minimize convection, such as counterdiffusion in polyvinyl alcohol gel coupled with the influence of a 500MHz (10.2T) magnetic field, were necessary to isolate the poorest diffracting polymorph and increase its internal order to determine its structure by X-ray diffraction. The results obtained show the effectiveness of nonconventional crystallographic methods to isolate different crystal polymorphs. © 2015 International Union of Crystallography.
Filiaciones:
De La Mora E.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, Mexico City 04510, DF, Mexico
Departamento de Quýmica de Biomacromoleculas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Avenida Universidad 3000, Coyoacan, Mexico D.F., 04510, Mexico
Flores-Hernández E.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, Mexico City 04510, DF, Mexico
Departamento de Quýmica de Biomacromoleculas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Avenida Universidad 3000, Coyoacan, Mexico D.F., 04510, Mexico
Jakoncic J.:
National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, United States
Stojanoff V.:
National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY, United States
Siliqi D.:
Istituto di Cristallografia, Consiglio Nazionale Delle Ricerche, Via G. Amendola 122/O70122, Italy
Sánchez-Puig N.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, Mexico City 04510, DF, Mexico
Departamento de Quýmica de Biomacromoleculas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Avenida Universidad 3000, Coyoacan, Mexico D.F., 04510, Mexico
Moreno A.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, Mexico City 04510, DF, Mexico
Departamento de Quýmica de Biomacromoleculas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Avenida Universidad 3000, Coyoacan, Mexico D.F., 04510, Mexico
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