acal is a Long Non-coding RNA in JNK Signaling in Epithelial Shape Changes during Drosophila Dorsal Closure


Por: Ríos-Barrera L.D., Gutiérrez-Pérez I., Domínguez M., RiesgoEscovar, R

Publicada: 1 feb 2015
Resumen:
Dorsal closure is an epithelial remodeling process taking place during Drosophila embryogenesis. JNK signaling coordinates dorsal closure. We identify and characterize acal as a novel negative dorsal closure regulator. acal represents a new level of JNK regulation. The acal locus codes for a conserved, long, non-coding, nuclear RNA. Long non-coding RNAs are an abundant and diverse class of gene regulators. Mutations in acal are lethal. acal mRNA expression is dynamic and is processed into a collection of 50 to 120 bp fragments. We show that acal lies downstream of raw, a pioneer protein, helping explain part of raw functions, and interacts genetically with Polycomb. acal functions in trans regulating mRNA expression of two genes involved in JNK signaling and dorsal closure: Connector of kinase to AP1 (Cka) and anterior open (aop). Cka is a conserved scaffold protein that brings together JNK and Jun, and aop is a transcription factor. Misregulation of Cka and aop can account for dorsal closure phenotypes in acal mutants. © 2015 Ríos-Barrera et al.

Filiaciones:
Ríos-Barrera L.D.:
 Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro, Mexico

Gutiérrez-Pérez I.:
 Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, Campus de San Juan, Sant Joan d’Alacant, Alicante, Spain

Domínguez M.:
 Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, Campus de San Juan, Sant Joan d’Alacant, Alicante, Spain

RiesgoEscovar, R:
 Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro, Mexico
ISSN: 15537390
Editorial
PUBLIC LIBRARY SCIENCE, 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 11 Número: 2
Páginas: 1-27
WOS Id: 000352081800013
ID de PubMed: 25710168
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