IL-1 beta increases necrotic neuronal cell death in the developing rat hippocampus after status epilepticus by activating type I IL-1 receptor (IL-1RI)
Por:
Medel-Matus, JS, Alvarez-Croda, DM, Martínez-Quiroz J., Beltrán-Parrazal L., Morgado-Valle C., Lopez-Meraz, ML
Publicada:
1 nov 2014
Resumen:
Interleukin-1 beta (IL-1 beta) is associated with seizure-induced neuronal cell death in the adult brain. The contribution of IL-1 beta to neuronal injury induced by status epilepticus (SE) in the immature brain remains unclear. In the present study, we investigated the effects of IL-1 beta administration on hippocampal neuronal cell death associated with SE in the immature brain, and the role of the type I receptor of IL-1 beta (IL-1RI). SE was induced with lithium-pilocarpine in 14-days-old (P14) rat pups. Six hours after SE onset, pups were i.c.v. injected in the right ventricle with IL-1 beta (0, 0.3, 3, 30, or 300 ng), 30 ng of IL-1RI antagonist (IL-1Ra) alone, or 30 ng of IL-1Ra plus 3 ng of IL-1 beta. As control groups, pups without seizures were injected with 3 ng of IL-1 beta or vehicle. Twenty-four hours after SE onset, neuronal cell death in the CA1 field of dorsal hippocampus was assessed by hematoxylin-eosin, Fluoro-Jade B and in vivo propidium iodide (PI) staining; expres
Filiaciones:
Martínez-Quiroz J.:
Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas-IPN, Wilfrido Massieu s/n Unidad Profesional Adolfo López Mateos, Gustavo A. Madero, México D.F., C.P. 07738, Mexico
Beltrán-Parrazal L.:
Centro de Investigaciones Cerebrales, Universidad Veracruzana, Médicos y Odontologos s/n, Col. Unidad del Bosque, Xalapa, Veracruz, C.P. 91010, Mexico
Morgado-Valle C.:
Centro de Investigaciones Cerebrales, Universidad Veracruzana, Médicos y Odontologos s/n, Col. Unidad del Bosque, Xalapa, Veracruz, C.P. 91010, Mexico
Doctorado en Neuroetología, Instituto de Neuroetología, Universidad Veracruzana, Av. Luis Castelazo s/n Carr. Industrial-Ánimas, Xalapa, Veracruz, C.P. 91190, Mexico
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