The mitochondrial respiratory chain of the secondary green alga Euglena gracilis shares many additional subunits with parasitic Trypanosomatidae
Por:
Perez E., Lapaille M., Degand H., Cilibrasi L., Villavicencio-Queijeiro A., Morsomme P., González-Halphen D., Field M.C., Remacle C., Baurain D., Cardol P.
Publicada:
1 nov 2014
Resumen:
The mitochondrion is an essential organelle for the production of
cellular ATP in most eukaryotic cells. It is extensively studied,
including in parasitic organisms such as trypanosomes, as a potential
therapeutic target. Recently, numerous additional subunits of the
respiratory-chain complexes have been described in Trypanosoma brucei
and Dypanosoma cruzi. Since these subunits had apparently no
counterparts in other organisms, they were interpreted as potentially
associated with the parasitic trypanosome lifestyle. Here we used two
complementary approaches to characterise the subunit composition of
respiratory complexes in Euglena gracilis, a non-parasitic secondary
green alga related to trypanosomes. First, we developed a phylogenetic
pipeline aimed at mining sequence databases for identifying homologues
to known respiratory-complex subunits with high confidence. Second, we
used MS/MS proteomics after two-dimensional separation of the
respiratory complexes by Blue Native- and SOS-PAGE both to confirm in
silico predictions and to identify further additional subunits.
Altogether, we identified 41 subunits that are restricted to E.
gracilis, T. brucei and T. cruzi, along with 48 classical subunits
described in other eukaryotes (i.e. plants, mammals and fungi). This
moreover demonstrates that at least half of the subunits recently
reported in T. brucei and T. cruzi are actually not specific to
Trypanosomatidae, but extend at least to other Euglenozoa, and that
their origin and function are thus not specifically associated with the
parasitic lifestyle. Furthermore, preliminary biochemical analyses
suggest that some of these additional subunits underlie the
peculiarities of the respiratory chain observed in Euglenozoa. (C) 2014
Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Filiaciones:
Perez E.:
Genetics and Physiology of Microalgae, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
Eukaryotic Phylogenomics, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
Lapaille M.:
Genetics and Physiology of Microalgae, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
Degand H.:
Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium
Cilibrasi L.:
Genetics and Physiology of Microalgae, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
Villavicencio-Queijeiro A.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico
Morsomme P.:
Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium
González-Halphen D.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico
Field M.C.:
Division of Biological Chemistry and Drug Discovery, University of Dundee, Dundee, Scotland, DD1 5EH, United Kingdom
Remacle C.:
Genetics and Physiology of Microalgae, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
PhytoSYSTEMS, University of Liège, Liège, B-4000, Belgium
Baurain D.:
Eukaryotic Phylogenomics, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
PhytoSYSTEMS, University of Liège, Liège, B-4000, Belgium
Cardol P.:
Genetics and Physiology of Microalgae, Department of Life Sciences, University of Liège, Liège, B-4000, Belgium
PhytoSYSTEMS, University of Liège, Liège, B-4000, Belgium
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