Age-dependent increment of hydroxymethylation in the brain cortex in the triple-transgenic mouse model of Alzheimer's disease
Por:
Cadena-del-Castilloa, C, Valdes-Quezada, C, Carmona-Aldana, F, Arias, C, Bermudez-Rattoni, F, Recillas-Targa, F
Publicada:
1 ene 2014
Resumen:
Alzheimer's disease (AD) is a complex disorder whose etiology is associated with environmental and genetic factors. Recently there have been several attempts to analyze the role of epigenetic alterations in the origin and progression of this neurodegenerative condition. To evaluate the potential participation of the methylation status of the genome that may contribute to AD progression, we have studied the levels and distribution of the 5-methylcytosine and 5-hydroxymethylcytosine in different brain regions at different ages.We analyzed and quantified the immunosignal of these two epigenetic marks in young versus old wild-type mice and in the triple-transgenic mouse model of AD(3xTg-AD). The results showa decline in global 5-methylcytosine mark over time in all studied brain regions concomitant with a significant and widespread increase in 5-hydroxymethylcytosine mark in the aged transgenic mice in contrast to the age-matched controls. These differences in the methylation pattern of brain DNA in the 3xTg-AD that accumulates along age indicates abnormal formation of permissive chromatin structure associated with the increase in AD-related markers. © 2014-IOS Press and the authors. All rights reserved.
Filiaciones:
Cadena-del-Castilloa, C:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Valdes-Quezada, C:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Carmona-Aldana, F:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Arias, C:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Med Genom & Toxicol Ambiental, Mexico City 04510, DF, Mexico
Bermudez-Rattoni, F:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Mexico City 04510, DF, Mexico
Recillas-Targa, F:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
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