Anterior and intermediate pituitary tissues express claudin 4 in follicle stellate cells and claudins 2 and 5 in endothelial cells


Por: García-Godínez A., Contreras R.G., González-Del-Pliego M., Aguirre-Benítez E., Acuna-Macias, I, De La Vega M.T., Martín-Tapia D., Solano-Agama C., Mendoza-Garrido M.E.

Publicada: 1 jul 2014
Resumen:
Follicle-stellate cells are pituitary non-granular cells that are arranged between secretory cells or organized in follicles with small lumens. Cells from the follicles exhibit the typical phenotype of a transporting epithelium, including apical microvilli with a cilium and tight junctions. Freeze-fracture electron microscopy images show that the tight junctions consist of 5-7 anastomosing strands and that cultured follicle-stellate cells develop a trans-epithelial electrical resistance characteristic of "tight" epithelia. Here, we investigate the molecular composition of the tight junction from follicle stellate cells. We found that the rat anterior pituitary lobe expresses mRNAs for claudins 2, 4 and 5; the proteins of all these claudins are observed in the anterior lobe, whereas the intermediate lobe expresses claudins 2 and 5 and the posterior lobe contains only claudin 5. Follicle-stellate cells, identified by their protein marker S100ß, expresses claudin 4 in the apical membrane, in co-localization with dipeptidyl-peptidase and near acetylated ß-tubulin. Claudin 4 partially co-localizes with E-cadherin, indicating that a fraction of the protein is located in the basolateral domain. Follicle-stellate-enriched cell cultures develop patches of polygonal cells expressing claudin 4 and E-cadherin, encircled by extensive monolayers of fusiform cells. Claudin 2 stains specifically blood vessels, identified by claudin 5 and VE-cadherin labels. Thus, follicles in the anterior pituitary consist of "tight" epithelia that can carry out intense vectorial transport, together with a high cation movement in blood vessels, possibly related to the ion requirements of excitable secretory cells for hormone secretion. © 2014 Springer-Verlag.

Filiaciones:
García-Godínez A.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

Contreras R.G.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

González-Del-Pliego M.:
 Univ Nacl Autonoma Mexico, Dept Embryol, Fac Med, Mexico City, DF, Mexico

Aguirre-Benítez E.:
 Univ Nacl Autonoma Mexico, Dept Embryol, Fac Med, Mexico City, DF, Mexico

De La Vega M.T.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

Martín-Tapia D.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

Solano-Agama C.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

Mendoza-Garrido M.E.:
 Department of Physiology, Biophysics and Neuroscience, Cinvestav-IPN, Mexico City, Mexico

 Department of Physiology, Biophysics and Neuroscience, CINVESTAV-IPN, Av. IPN No. 2508 Col. San Pedro Zacatenco, Mexico City, CP 07360, Mexico
ISSN: 0302766X
Editorial
Springer-Verlag, 233 SPRING ST, NEW YORK, NY 10013 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 357 Número: 1
Páginas: 309-321
WOS Id: 000338759900026
ID de PubMed: 24760107