Cystatin C has a dual role in post-traumatic brain injury recovery
Por:
Martinez-Vargas, M, Soto-Nunez, M, Tabla-Ramon, E, Solis, B, Gonzalez-Rivera, R, Perez-Arredondo, A, Estrada-Rojo, F, Castell, A, Molina-Guarneros, J, Navarro, L
Publicada:
1 abr 2014
Resumen:
Cathepsin B is one of the major lysosomal cysteine proteases involved in neuronal protein catabolism. This cathepsin is released after traumatic injury and increases neuronal death; however, release of cystatin C, a cathepsin inhibitor, appears to be a self-protective brain response. Here we describe the effect of cystatin C intracerebroventricular administration in rats prior to inducing a traumatic brain injury. We observed that cystatin C injection caused a dual response in post-traumatic brain injury recovery: higher doses (350 fmoles) increased bleeding and mortality, whereas lower doses (3.5 to 35 fmoles) decreased bleeding, neuronal damage and mortality. We also analyzed the expression of cathepsin B and cystatin C in the brains of control rats and of rats after a traumatic brain injury. Cathepsin B was detected in the brain stem, cerebellum, hippocampus and cerebral cortex of control rats. Cystatin C was localized to the choroid plexus, brain stem and cerebellum of control rats. Twenty-four hours after traumatic brain injury, we observed changes in both the expression and localization of both proteins in the cerebral cortex, hippocampus and brain stem. An early increase and intralysosomal expression of cystatin C after brain injury was associated with reduced neuronal damage. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Filiaciones:
Martinez-Vargas, M:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Soto-Nunez, M:
Univ Nacl Autonoma Mexico, Sch Med, Dept Pharmacol, Mexico City 04510, DF, Mexico
Tabla-Ramon, E:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Solis, B:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Gonzalez-Rivera, R:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Perez-Arredondo, A:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Estrada-Rojo, F:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
Castell, A:
Univ Nacl Autonoma Mexico, Sch Med, Dept Cell & Tissue Biol, Mexico City 04510, DF, Mexico
Molina-Guarneros, J:
Univ Nacl Autonoma Mexico, Sch Med, Dept Pharmacol, Mexico City 04510, DF, Mexico
Navarro, L:
Univ Nacl Autonoma Mexico, Sch Med, Dept Physiol, Mexico City 04510, DF, Mexico
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