Respiratory syncytial virus persistence in macrophages upregulates fcgamma receptors expression
Por:
Gaona, J, Santiago-Olivares, C, Ortega, E, Gomez, B
Publicada:
1 feb 2014
Resumen:
Viruses can persist in differentiated cells (i.e., macrophages) over long periods of time, altering host cells functions but not inducing their death. We had previously reported that, in early passages (14-40) of a murine macrophage-like cell line persistently infected with respiratory syncytial virus (RSV) (MfP), Fc?R-mediated phagocytosis and expression of Fc?RIIB/RIII on the cell membrane were increased with respect to mock-infected macrophages (MfN). In this work, we explored the mechanism underlying such effects. Increases in Fc?R expression and Fc?R-mediated phagocytosis are preserved after more than 87 passages of the persistently infected culture. We analyzed the expression of Fc?R isoforms at both mRNA and protein levels, and found out that RSV persistence distinctly affects the expression of Fc?R isoforms. We also observed that the increase in Fc?Rs expression results neither from soluble factors (cytokines) or viral products released by the infected cells, nor from an increase in the rate of Fc?R internalization. Our results suggest that RSV persistence in macrophages induce intracellular effects that have an impact on Fc?Rs gene expression at both mRNA and protein levels, and that the characteristics of RSV persistence were preserved for over 87 passages. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Filiaciones:
Gaona, J:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Santiago-Olivares, C:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Ortega, E:
Univ Nacl Autonoma Mexico, Dept Immunol, Biomed Res Inst, Mexico City 04510, DF, Mexico
Gomez, B:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
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