Biosynthesis of poly-ß-hydroxybutyrate (PHB) with a high molecular mass by a mutant strain of Azotobacter vinelandii (OPN)


Por: Pena, C, Lopez, S, Garcia, A, Espin, G, Romo-Uribe, A, Segura, D

Publicada: 1 mar 2014
Categoría: Applied Microbiology and Biotechnology

Resumen:
The aim of this study was to characterize the influence of the aeration conditions on the production of PHB and its molecular mass in a mutant strain of Azotobacter vinelandii (OPN), which carries a mutation on ptsN, the gene encoding enzyme IIANtr, previously shown to increase the accumulation of PHB. Cultures of A. vinelandii wild-type strain OP and its mutant derivative strain OPN were grown in 500-mL flasks, containing 100 and 200 mL of PY sucrose medium. PHB production and its molecular mass were analyzed at the end of the culture. The molecular mass (MM) was significantly influenced by the aeration conditions and strain used. A polymer with a higher molecular weight was produced under low aeration conditions for both strains. A maximal molecular mass of 2,026 kDa (equivalent to 3,670 kDa measured by GPC) was obtained with strain OPN cultured under low-aeration conditions, reaching a value two-fold higher than that obtained from the parental strain OP (MM = 1,013 kDa) grown under the same conditions. Aeration conditions and the ptsN mutation influence the molecular mass of the PHB produced by A. vinelandii affecting in turn its physico-chemical properties. © 2013 Springer-Verlag Berlin Heidelberg and the University of Milan.

Filiaciones:
Pena, C:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62210, Morelos, Mexico

Lopez, S:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62210, Morelos, Mexico

Garcia, A:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62210, Morelos, Mexico

Espin, G:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Microbiol Mol, Cuernavaca 62210, Morelos, Mexico

Romo-Uribe, A:
 Univ Nacl Autonoma Mexico, Inst Ciencias Fis, Lab Nanopolimeros & Coloides, Cuernavaca 62210, Morelos, Mexico

Segura, D:
 Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Microbiol Mol, Cuernavaca 62210, Morelos, Mexico
ISSN: 15904261
Editorial
SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 64 Número: 1
Páginas: 39-47
WOS Id: 000331648300005