High Performance Liquid Chromatography Fluorescence Method for the Determination of Seleno-Amino Acids in Ovine Blood Plasma
Por:
Valdez-Flores C., Revilla-Vázquez A.L., Ramírez-Bribiesca J.E., López-Arellano R., Pérez-Becerril E.F.
Publicada:
11 feb 2014
Resumen:
This study was performed to validate an effective high performance liquid chromatography-fluorescence detection method for the separation of seleno-amino acids and sulfur amino acids in biological samples using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Standard calibration and standard addition procedures were developed, but the quantification of seleno-amino acids was carried out by a second method. The coefficient of variation for precision was < 15% and accuracies were 96 to 98%. Recoveries in the extraction method were between 63 and 99%. This method was applied to the contribution of seleno-amino acids containing methylselenocysteine and selenomethionine in plasma tissue of lambs during infusion study. The method demonstrated good selectivity, sensitivity, precision, and accuracy. © 2014 Copyright Taylor and Francis Group, LLC.
Filiaciones:
Valdez-Flores C.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Toluca, Mexico
Revilla-Vázquez A.L.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Toluca, Mexico
Ramírez-Bribiesca J.E.:
Colegio de Postgraduados, Enlace Ganadería, Montecillo, Texcoco, Mexico
López-Arellano R.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Toluca, Mexico
Pérez-Becerril E.F.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Toluca, Mexico
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