Pre-Exposure of Mycobacterium tuberculosis-Infected Macrophages to Crystalline Silica Impairs Control of Bacterial Growth by Deregulating the Balance between Apoptosis and Necrosis
Por:
Chávez-Galán L., Ramon-Luing L.A., Torre-Bouscoulet L., Pérez-Padilla R., Sada-Ovalle I.
Publicada:
22 nov 2013
Resumen:
Inhalation of crystalline silica (CS) particles increases the risk of pulmonary tuberculosis; however, the precise mechanism through which CS exposure facilitates Mycobacterium tuberculosis (Mtb) infection is unclear. We speculate that macrophage exposure to CS deregulates the cell death pathways that could explain, at least in part, the association observed between exposure to CS and pulmonary tuberculosis. We therefore established an in vitro model in which macrophages were exposed to CS and then infected with Mtb. Expression of surface markers was analyzed by flow cytometry, JNK1/2, ASK1, caspase 9, P-p38, Bcl-2 and Mcl-1 were analyzed by Western blot, and cytokines by ELISA. Our results show that exposure to CS limits macrophage ability to control Mtb growth. Moreover, this exposure reduced the expression of TLR2, Bcl-2 and Mcl-1, but increased that of JNK1 and ASK1 molecules in the macrophages. Finally, when the pre-exposed macrophages were infected with Mtb, the concentrations of
Filiaciones:
Chávez-Galán L.:
Laboratory of Integrative Immunology, National Institute of Respiratory Diseases Ismael Cosío Villegas, Mexico City, Mexico
Ramon-Luing L.A.:
Laboratory of Integrative Immunology, National Institute of Respiratory Diseases Ismael Cosío Villegas, Mexico City, Mexico
Torre-Bouscoulet L.:
Department of Respiratory Physiology, National Institute of Respiratory Diseases Ismael Cosío Villegas, Mexico City, Mexico
Pérez-Padilla R.:
Department of Respiratory Physiology, National Institute of Respiratory Diseases Ismael Cosío Villegas, Mexico City, Mexico
Sada-Ovalle I.:
Laboratory of Integrative Immunology, National Institute of Respiratory Diseases Ismael Cosío Villegas, Mexico City, Mexico
Gold
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