Mechanical and structural response of a hybrid hydrogel based on chitosan and poly(vinyl alcohol) cross-linked with epichlorohydrin for potential use in tissue engineering
Por:
Garnica-Palafox I.M., Sánchez-Arévalo F.M., Velasquillo C., García-Carvajal Z.Y., García-López J., Ortega-Sánchez C., Ibarra C., Luna-Bárcenas G., Solís-Arrieta L.
Publicada:
2 ene 2014
Resumen:
The development and characterization of a hybrid hydrogel based on chitosan (CS) and poly(vinyl alcohol) (PVA) chemically cross-linked with epichlorohydrin (ECH) is presented. The mechanical response of these hydrogels was evaluated by uniaxial tensile tests; in addition, their structural properties such as average molecular weight between cross-link points (Mcrl), mesh size (DN), and volume fraction (vs) were determined. This was done using the equivalent polymer network theory in combination with the obtained results from tensile and swelling tests. The films showed Youngs modulus values of 11 ± 2 MPa and 9 ± 1 MPa for none irradiated and ultraviolet (UV) irradiated hydrogels, respectively. The cell viability was assessed using Calcein AM and Ethidium homodimer-1 assay and environmental scanning electron microscopy. The 1-(4,5-dimethylthiazol-2-yl)-3,5- diphenylformazan thiazolyl blue formazan (MTT Formazan assay) results did not show cytotoxic effects; this was in good agreement with nuclear magnetic resonance and fourier transform infrared spectroscopies; their results did not show traces of ECH. This indicated that after the crosslinking process, there was no free ECH; furthermore, any possibility of ECH release in the construct during cell culture was discarded. The CS-PVA-ECH hybrid hydrogel allowed cell growth and extracellular matrix formation and showed adequate mechanical, structural, and biological properties for potential use in tissue engineering applications. © 2013 Taylor & Francis.
Filiaciones:
Garnica-Palafox I.M.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04360, DF, Mexico
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, Cd. Universitaria, Apdo. Postal 70-360, México D.F., 04360, Mexico
Sánchez-Arévalo F.M.:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04360, DF, Mexico
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, Cd. Universitaria, Apdo. Postal 70-360, México D.F., 04360, Mexico
Velasquillo C.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
García-Carvajal Z.Y.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
García-López J.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
Ortega-Sánchez C.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
Ibarra C.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
Luna-Bárcenas G.:
Polymer and Biopolymer Research Group, CINVEST Querétaro, México D.F., Mexico
Solís-Arrieta L.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Biotecnologia Instituto Nacional de Rehabilitacion, México D.F., Mexico
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