HLA Class I and Class II Conserved Extended Haplotypes and Their Fragments or Blocks in Mexicans: Implications for the Study of Genetic Diversity in Admixed Populations
Por:
Zuniga, J, Yu N., Barquera R., Alosco S., Ohashi M., Lebedeva T., Acuna-Alonzo, V, Yunis M., Granados-Montiel J., Cruz-Lagunas A., Vargas-Alarcón G., Rodríguez-Reyna T.S., Fernandez-Viña M., Granados J., Yunis E.J.
Publicada:
23 sep 2013
Resumen:
Major histocompatibility complex (MHC) genes are highly polymorphic and informative in disease association, transplantation, and population genetics studies with particular importance in the understanding of human population diversity and evolution. The aim of this study was to describe the HLA diversity in Mexican admixed individuals. We studied the polymorphism of MHC class I (HLA-A, -B, -C), and class II (HLA-DRB1, -DQB1) genes using high-resolution sequence based typing (SBT) method and we structured the blocks and conserved extended haplotypes (CEHs) in 234 non-related admixed Mexican individuals (468 haplotypes) by a maximum likelihood method. We found that HLA blocks and CEHs are primarily from Amerindian and Caucasian origin, with smaller participation of African and recent Asian ancestry, demonstrating a great diversity of HLA blocks and CEHs in Mexicans from the central area of Mexico. We also analyzed the degree of admixture in this group using short tandem repeats (STRs) and HLA-B that correlated with the frequency of most probable ancestral HLA-C/-B and -DRB1/-DQB1 blocks and CEHs. Our results contribute to the analysis of the diversity and ancestral contribution of HLA class I and HLA class II alleles and haplotypes of Mexican admixed individuals from Mexico City. This work will help as a reference to improve future studies in Mexicans regarding allotransplantation, immune responses and disease associations. © 2013 Zuñiga et al.
Filiaciones:
Yu N.:
HLA Laboratory, The American Red Cross Northeast Division, Dedham, MA, United States
Barquera R.:
Molecular Genetics Laboratory, National School of Anthropology and History, Mexico City, Mexico
Alosco S.:
HLA Laboratory, The American Red Cross Northeast Division, Dedham, MA, United States
Ohashi M.:
HLA Laboratory, The American Red Cross Northeast Division, Dedham, MA, United States
Lebedeva T.:
HLA Laboratory, The American Red Cross Northeast Division, Dedham, MA, United States
Yunis M.:
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States
Granados-Montiel J.:
Tissue Engineering, Cell Therapy and Regenerative Medicine Research Unit, Instituto Nacional de Rehabilitación, Mexico City, Mexico
Cruz-Lagunas A.:
Department of Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico
Vargas-Alarcón G.:
Laboratory of Genomics, Instituto Nacional de Cardiología Ignacio Chavez, Mexico City, Mexico
Rodríguez-Reyna T.S.:
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Fernandez-Viña M.:
Department of Pathology, Stanford University, Stanford, CA, United States
Granados J.:
Department of Transplantation, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
Yunis E.J.:
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States
Gold
|