Differential DNA methylation pattern in the A and B promoters of the progesterone receptor is associated with differential mRNA expression in the female rat hypothalamus during proestrus
Por:
Mendoza-Garcés L., Rodríguez-Dorantes M., Álvarez-Delgado C., Vázquez-Martínez E.R., Garcia-Tobilla P., Cerbón M.A.
Publicada:
16 oct 2013
Resumen:
In rodents, the display of reproductive behavior occurs during the
proestrus-estrus transition of the estrus cycle. This behavior is
regulated by estradiol and progesterone mainly via their intracellular
receptors. Two isoforms of the progesterone receptor have been described
(A and B), and they have different promoters for their regulation. It
has been demonstrated that the mRNA for both isoforms changes during the
proestrus-estrus transition. It has been recently established that DNA
methylation can be transient and cyclical in gene promoters, however,
these changes have only been reported in vitro but not in physiological
models. The aim of this study was to analyze the pattern of DNA
methylation in the PR (A and B) promoter regions during the
proestrus-estrus transition in the rat hypothalamus and its correlation
with the regulation of mRNA expression. The results demonstrated a
differential mRNA expression of the progesterone receptor (A and B)
isoforms. The expression of total PR did not change significantly during
the proestrus day, while the expression of isoform B increased
significantly at 17:00 h, followed by a significant decrease at 21:00 h
of the proestrus day. Interestingly, we also found that the isoform A
promoter was mainly unmethylated at all studied time points. In
contrast, the isoform B promoter showed a transient methylation increase
during the evening of proestrus. The overall results indicate that there
is a switch of progesterone receptor isoforms expression during the
evening of proestrus that is related to the differential gene
methylation patterns of their promoter regions, mainly for the isoform B
promoter. (C) 2013 Elsevier B.V. All rights reserved.
Filiaciones:
Mendoza-Garcés L.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Rodríguez-Dorantes M.:
Instituto Nacional de Medicina Genómica, Periférico Sur 4809 Arenal Tepepan, Tlalpan, 14610 México, D.F., Mexico
Álvarez-Delgado C.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Vázquez-Martínez E.R.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Garcia-Tobilla P.:
Instituto Nacional de Medicina Genómica, Periférico Sur 4809 Arenal Tepepan, Tlalpan, 14610 México, D.F., Mexico
Cerbón M.A.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
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