Transcript profiling of common bean nodules subjected to oxidative stress
Por:
Ramírez M., Guillén G., Fuentes S.I., Iniguez, LP, Aparicio-Fabre R., Zamorano-Sánchez D., Encarnación-Guevara S., Panzeri D., Castiglioni B., Cremonesi P., Strozzi F., Stella A., Girard L., Sparvoli F., Hernández G.
Publicada:
1 nov 2013
Resumen:
Several environmental stresses generate high amounts of reactive oxygen species (ROS) in plant cells, resulting in oxidative stress. Symbiotic nitrogen fixation (SNF) in the legume-rhizobia symbiosis is sensitive to damage from oxidative stress. Active nodules of the common bean (Phaseolus vulgaris) exposed to the herbicide paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride hydrate), which stimulates ROS accumulation, exhibited reduced nitrogenase activity and ureide content. We analyzed the global gene response of nodules subjected to oxidative stress using the Bean Custom Array 90K, which includes probes from 30000 expressed sequence tags (ESTs). A total of 4280 ESTs were differentially expressed in stressed bean nodules; of these, 2218 were repressed. Based on Gene Ontology analysis, these genes were grouped into 42 different biological process categories. Analysis with the PathExpress bioinformatic tool, adapted for bean, identified five significantly repressed metabolic pathways related to carbon/nitrogen metabolism, which is crucial for nodule function. Quantitative reverse transcription (qRT)-PCR analysis of transcription factor (TF) gene expression showed that 67 TF genes were differentially expressed in nodules exposed to oxidative stress. Putative cis-elements recognized by highly responsive TF were detected in promoter regions of oxidative stress regulated genes. The expression of oxidative stress responsive genes and of genes important for SNF in bacteroids analyzed in stressed nodules revealed that these conditions elicited a transcriptional response. © 2013 Scandinavian Plant Physiology Society.
Filiaciones:
Ramírez M.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Guillén G.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62209, Morelos, Mexico
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Mor. 62209, Mexico
Fuentes S.I.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Iniguez, LP:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Aparicio-Fabre R.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Zamorano-Sánchez D.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Encarnación-Guevara S.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Panzeri D.:
Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, Milano, 20133, Italy
Castiglioni B.:
Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, Milano, 20133, Italy
Cremonesi P.:
Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, Milano, 20133, Italy
Strozzi F.:
Parco Tecnologico Padano, Statistical Genomics and Bioinformatics Unit, Via Einstein Loc. Cascina Codazza, Lodi, Italy
Stella A.:
Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, Milano, 20133, Italy
Parco Tecnologico Padano, Statistical Genomics and Bioinformatics Unit, Via Einstein Loc. Cascina Codazza, Lodi, Italy
Girard L.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
Sparvoli F.:
Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, Milano, 20133, Italy
Hernández G.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62209, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 1001, Cuernavaca, Mor. 62209, Mexico
|