O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin
Por:
Jiménez-Martínez M.C., Lascurain R., Méndez-Reguera A., Estrada-Parra S., Estrada-García I., Gorocica P., Martínez-Cairo S., Zenteno E., Chávez R.
Publicada:
1 ene 2013
Resumen:
O-glycosidically-linked glycans have been involved in development,
maturation, homing, and immune regulation in T cells. Previous reports
indicate that Amaranthus leucocarpus lectin (ALL), specific for glycans
containing galactose-N-acetylgalactosamine and N-acetylgalactosamine,
recognizes human naive CD27(+)CD25(+)CD4(+) T cells. Our aim was to
evaluate the phenotype of CD4(+) T cells recognized by ALL in peripheral
blood mononuclear cells obtained from healthy volunteers. CD4(+) T cells
were isolated by negative selection using magnetic beads-labeled
monoclonal antibodies; the expression of T regulatory cell phenotypic
markers was assessed on ALL-recognized cells. In addition, IL-4, IL-10,
IFN-gamma, and TGF-beta intracellular production in ALL(+) cells was
also evaluated. The analyses of phenotypic markers and intracellular
cytokines were performed through flow cytometry. ALL-recognized CD4(+) T
cells were mainly CD45RA(+), CCR7(+) cells. Although 52 +/- 10%
CD25(+)Foxp3(+) cells were positive to ALL, only 34 +/- 4% of ALL(+)
cells corresponded to CD25(+)Foxp3(-) cells. Intracellular cytokines in
freshly obtained ALL(+)CD4(+) T cells exhibited 8% of IL-4, 15% of
IL-10, 2% of IFN-gamma, and 15% of TGF-beta, whereas ALL CD4(+) T
cells depicted 1% of IL-4, 2% of IL-10, <1% of IFN-gamma, and 6% of
TGF-beta Our results show that galactose-N-acetylgalactosamine and
N-galactosamine-bearing CD4(+) T cells expressed phenotypic markers of
NnTreg cells.
Filiaciones:
Jiménez-Martínez M.C.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Lab Inmunol, Mexico City 04510, DF, Mexico
Lascurain R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Lab Inmunol, Mexico City 04510, DF, Mexico
Méndez-Reguera A.:
Unidad de Investigación, Instituto de Oftalmología Fundación Conde de Valenciana, 06800 Mexico, DF, Mexico
Estrada-Parra S.:
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, 11340 Mexico, DF, Mexico
Estrada-García I.:
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, 11340 Mexico, DF, Mexico
Gorocica P.:
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 4502 Mexico, DF, Mexico
Martínez-Cairo S.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Lab Inmunol, Mexico City 04510, DF, Mexico
Zenteno E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Lab Inmunol, Mexico City 04510, DF, Mexico
Chávez R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Lab Inmunol, Mexico City 04510, DF, Mexico
Gold
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