Endothelial cell invasion by Toxoplasma gondii: differences between cell types and parasite strains
Por:
Canedo-Solares, I, Calzada-Ruiz M., Ortiz-Alegría L.B., Ortiz-Muñiz A.R., Correa D.
Publicada:
1 ago 2013
Resumen:
Toxoplasma gondii disseminates and causes congenital infection by invasion of the endothelial cells. The aim of this study was to analyze the ability of two strains to invade two endothelial cell types. Tachyzoites of the RH and ME49 strains were expanded in Balb/c and C57BL6-RAG2-/- mice, respectively. Tachyzoites were harvested from 72 h Vero cell cultures and incubated for 30 min to 4 h at 10:1 parasite/cell ratio in 24-well plates, containing monolayers of either HMEC-1 line or human umbilical cells (HUVECs). The number of infected cells and parasitic vacuoles per infected cell were counted in Wright stained slides. A slow increase in the proportion of infected cells occurred but varied according to cell type-parasite strain combination: ME49 tachyzoites invaded up to 63 % HMEC-1 cells, while RH parasites infected up to 19 % HUVECs. ME49 and RH tachyzoites invaded 49 and 46 % HUVECs and HMEC-1 cells, respectively. Reinvasion and formation of new parasitophorous vacuoles of infected
Filiaciones:
Calzada-Ruiz M.:
Laboratorio de Inmunología Experimental, Subdirección de Medicina Experimental, Instituto Nacional de Pediatría Secretaría de Salud, Mexico Distrito Federal, Mexico
Ortiz-Alegría L.B.:
Laboratorio de Inmunología Experimental, Subdirección de Medicina Experimental, Instituto Nacional de Pediatría Secretaría de Salud, Mexico Distrito Federal, Mexico
Ortiz-Muñiz A.R.:
Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Mexico Distrito Federal, Mexico
Correa D.:
Laboratorio de Inmunología Experimental, Subdirección de Medicina Experimental, Instituto Nacional de Pediatría Secretaría de Salud, Mexico Distrito Federal, Mexico
Col. Insurgentes Cuicuilco, Av. Insurgentes Sur 3700-C, 04530 Mexico Distrito Federal, Mexico
Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Mexico Distrito Federal, Mexico
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