Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells
Por:
Monroy-Estrada, HI, Chirino, YI, Soria-Mercado, IE, Sanchez-Rodriguez, J
Publicada:
7 may 2013
Resumen:
Background: Lung cancer causes 1.4 million deaths worldwide while
non-small-cell lung cancer (NSCLC) represents 80-85% of the cases.
Cisplatin is a standard chemotherapy against this type of cancer;
however, tumor cell resistance to this drug limits its efficacy. Sea
anemones produce compounds with pharmacological activities that may be
useful for augmenting cisplatin efficacy. This study aimed to evaluate
the pharmacological activities of crude venom (CV) from the sea anemone
Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4)
to test their increase efficiency cisplatin cytotoxicity in human lung
adenocarcinoma cells.
Results: Pre-exposure to CV, F1 and F2 fractions increases cisplatin
cytotoxicity in human lung adenocarcinoma cells under specific
conditions. Exposure to CV at 50 mu gmL(-1) induced a reduction of
approximately 50% in cell viability, while a similar cytotoxic effect
was observed when cell culture was exposed to F1 at 25 mu gmL(-1) or F2
at 50 mu gmL(-1). The cell culture exposure to F1 (10 mu gmL(-1))
fraction combined with cisplatine (25 mu M) provoked a decrease in MTT
reduction until 65.57% while F2 (25 mu gmL(-1)) fraction combined with
cisplatin (10 mu M) provoked a decrease in MTT reduction of 72.55%.
Conclusions: The F1 fraction had the greatest effect on the lung
adenocarcinoma cell line compared with CV and F2. The combination of
antineoplastic drugs and sea anemone toxins might allow a reduction of
chemotherapeutic doses and thus mitigate side effects.
Filiaciones:
Monroy-Estrada, HI:
Univ Nacl Autonoma Mexico, Inst Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifiales, Puerto Morelos, Quintana Roo St, Mexico
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Tlalnepantla, Estado Do Mexic, Mexico
Univ Autonoma Baja California, Fac Ciencias Marinas, Ensenada, Baja California, Mexico
Chirino, YI:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Tlalnepantla, Estado Do Mexic, Mexico
Soria-Mercado, IE:
Univ Autonoma Baja California, Fac Ciencias Marinas, Ensenada, Baja California, Mexico
Sanchez-Rodriguez, J:
Univ Nacl Autonoma Mexico, Inst Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifiales, Puerto Morelos, Quintana Roo St, Mexico
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