Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
Por:
Rodríguez-Arellanes G., de Sousa C.N., de Medeiros-Muniz M., Ramírez J.A., Pizzini C.V., de Abreu-Almeida M., de Oliveira, MME, Fusco-Almeida, AM, Vite-Garín T., Pitangui N.S., Estrada-Bárcenas D.A., González-González A.E., Mendes-Giannini, MJS, Zancopé-Oliveira R.M., Taylor, ML
Publicada:
1 jul 2013
Resumen:
The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+mating type) and G-186AR (-mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective+ and-mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources. © 2013, American Society for Microbiology. All Rights Reserved.
Filiaciones:
Rodríguez-Arellanes G.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
de Sousa C.N.:
Instituto de Pesquisa Clínica Evandro Chagas, Fundacão Oswaldo Cruz, Rio de Janeiro, Brazil
de Medeiros-Muniz M.:
Instituto de Pesquisa Clínica Evandro Chagas, Fundacão Oswaldo Cruz, Rio de Janeiro, Brazil
Ramírez J.A.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
Pizzini C.V.:
Instituto de Pesquisa Clínica Evandro Chagas, Fundacão Oswaldo Cruz, Rio de Janeiro, Brazil
de Abreu-Almeida M.:
Instituto de Pesquisa Clínica Evandro Chagas, Fundacão Oswaldo Cruz, Rio de Janeiro, Brazil
Vite-Garín T.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
Pitangui N.S.:
Universidade Estadual Paulista, Araraquara, São Paulo, Brazil
Estrada-Bárcenas D.A.:
Colección Nacional de Cultivos Microbianos, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México DF, Mexico
González-González A.E.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
Zancopé-Oliveira R.M.:
Instituto de Pesquisa Clínica Evandro Chagas, Fundacão Oswaldo Cruz, Rio de Janeiro, Brazil
Taylor, ML:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol Parasitol, Mexico City 04510, DF, Mexico
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