Comparison of antioxidant activity of hydroethanolic fresh and aged garlic extracts and their effects on cerebral ischemia
Por:
Cervantes M.I., Balderas, PMD, Gutierrez-Banos, JD, Orozco-Ibarra M., Fernández-Rojas B., Medina-Campos O.N., Espinoza-Rojo M., Ruiz-Tachiquín M., Ortiz-Plata A., Salazar, MI, Rubio-Osornio M., Castaneda-Saucedo, E, Pedraza-Chaverri J., Calzada F., Aguilera P.
Publicada:
15 sep 2013
Resumen:
Antioxidant properties and protective effect of aged garlic extract
(AGE) and of 20% hydroethanolic fresh extracts from garlic clove (GCE)
and skin (GSE) on cerebral ischemia were evaluated by administering
extracts at the beginning of reperfusion in a rat model of stroke. All
three extracts scavenged superoxide anion, peroxynitrite anion, and
peroxyl radicals, but with different efficiencies; furthermore, GCE and
GSE scavenged hydroxyl radicals and GSE scavenged singlet oxygen. These
extracts significantly prevented reduction of neuronal nuclear antigen
in the infarcted area, although no improvement in neurological function
was observed. Importantly, GCE and GSE contained S-allylcystein, a
compound associated with AGE's neuroprotective effect against damage
induced by cerebral ischemia. Extracts decreased mRNA expression of NR1-
and NR2B-NMDA-receptor subunits and prevented ischemia-induced reduction
in mitochondrial potential and in ATP synthesis. These results indicate
that antioxidants present in garlic extracts may regulate ROS
concentrations during ischemia, favour pro-survival pathways, and
attenuate mitochondrial dysfunction. (C) 2013 Elsevier Ltd. All rights
reserved.
Filiaciones:
Cervantes M.I.:
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur #3877, Col. La Fama, México, D.F. C.P. 14269, Mexico
Unidad de Investigación Médica en Farmacología, Hospital de Especialidades 2 Piso-CORCE, Centro Médico Nacional Siglo XXI, Av. Cuauhtémoc #330, Col. Doctores, México, D.F. C.P. 06720, Mexico
Orozco-Ibarra M.:
Laboratorio de Neurobiología Molecular y Celular, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur #3877, Col. La Fama, México, D.F. C.P. 14269, Mexico
Fernández-Rojas B.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Medina-Campos O.N.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Espinoza-Rojo M.:
Laboratorio de Biología Molecular y Genómica, Universidad Autónoma de Guerrero, Ciudad Universitaria, Av. Lázaro Cárdenas s/n, Chilpancingo, Guerrero, C.P. 39087, Mexico
Ruiz-Tachiquín M.:
Unidad de Investigación Médica en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Av. Cuauhtémoc #330, Col. Doctores, México, D.F. C.P. 06720, Mexico
Ortiz-Plata A.:
Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur #3877, Col. La Fama, México, D.F. C.P. 14269, Mexico
Rubio-Osornio M.:
Laboratorio de Enfermedades Neurodegenerativas, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur #3877, Col. La Fama, México, D.F. C.P. 14269, Mexico
Pedraza-Chaverri J.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Calzada F.:
Unidad de Investigación Médica en Farmacología, Hospital de Especialidades 2 Piso-CORCE, Centro Médico Nacional Siglo XXI, Av. Cuauhtémoc #330, Col. Doctores, México, D.F. C.P. 06720, Mexico
Aguilera P.:
Laboratorio de Patología Vascular Cerebral, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur #3877, Col. La Fama, México, D.F. C.P. 14269, Mexico
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