Titanium dioxide nanoparticles inhibit proliferation and induce morphological changes and apoptosis in glial cells
Por:
Márquez-Ramírez S.G., Delgado-Buenrostro N.L., Chirino Y.I., Iglesias G.G., López-Marure R.
Publicada:
16 dic 2012
Categoría:
Toxicology
Resumen:
Titanium dioxide nanoparticles (TiO2 NPs) are widely used in the chemical, electrical and electronic industries. TiO2 NPs can enter directly into the brain through the olfactory bulb and be deposited in the hippocampus region. We determined the effect of TiO2 NPs on rat and human glial cells, C6 and U373, respectively. We evaluated proliferation by crystal violet staining, internalization of TiO2 NPs, and cellular morphology by TEM analysis, as well as F-actin distribution by immunostaining and cell death by detecting active caspase-3 and DNA fragmentation. TiO2 NPs inhibited proliferation and induced morphological changes that were related with a decrease in immuno-location of F-actin fibers. TiO2 NPs were internalized and formation of vesicles was observed. TiO2 NPs induced apoptosis after 96h of treatment. Hence, TiO2 NPs had a cytotoxic effect on glial cells, suggesting that exposure to TiO2 NPs could cause brain injury and be hazardous to health. (C) 2012 Elsevier Ireland Ltd. All
Filiaciones:
Márquez-Ramírez S.G.:
Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
Departamento de Biología Celular, Instituto Nacional de Cardiología 'Ignacio Chávez', Mexico
Delgado-Buenrostro N.L.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City, DF, Mexico
Chirino Y.I.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City, DF, Mexico
Iglesias G.G.:
Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico
López-Marure R.:
Departamento de Biología Celular, Instituto Nacional de Cardiología 'Ignacio Chávez', Mexico
|