Endogenous Content and Release of [H-3]-GABA and [H-3]-Glutamate in the Spinal Cord of Chronically Undernourished Rat
Por:
Quiroz-Gonzalez, S, Escartin-Perez, RE, Paz-Bermudez, F, Segura-Alegria, B, Reyes-Legorreta, C, Guadarrama-Olmos, JC, Floran-Garduno, B, Jimenez-Estrada, I
Publicada:
1 ene 2013
Resumen:
The aim of this study was to determine the effect of chronic
undernutrition on the content and release of gamma-amino butyric acid
(GABA) and glutamate (GLU) transmitters in the rat spinal cord. The
release of [H-3]-GABA and [H-3]-GLU was determined by radioactive
liquid scintillation techniques, and the concentrations of GABA and GLU
in spinal cord preparations from control and undernourished young rats
(50-60 days old) were measured by reverse-phase HPLC. The GABA and GLU
contents in the lumbar spinal dorsal horn (L6 segment) were
significantly lower in undernourished rats relative to control rats
(22.2 +/- A 3.7 and 10.7 +/- A 1.9 %, respectively; P < 0.05). Spinal
cord blocks from undernourished animals also showed lower rates of
[H-3]-GABA and [H-3]-GLU release than controls (27.6 +/- A 3.5 and
12.8 +/- A 2.5 %, respectively; P < 0.01). We propose that the
decreases in GLU content and release are consistent with a reduced
activation of either afferent fibers, spinal glutaminergic neurons, or
both. Furthermore, we propose that the decreased content and release of
GABA in undernourished animals are related to a depression in pre- and
post-synaptic inhibition. In addition, we hypothesize that the
reductions in GABA content and release serve as compensatory mechanisms
to counterbalance decreases in sensory transmission and GLU content in
the spinal cord of the chronically undernourished rat.
Filiaciones:
Escartin-Perez, RE:
Univ Nacl Autonoma Mexico, Lab Neurobiol Alimentac, FES Iztacala, Mexico City, DF, Mexico
Segura-Alegria, B:
Univ Nacl Autonoma Mexico, Fac Estudios Super, FES Iztacala, Mexico City, DF, Mexico
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