Oleamide restores sleep in adult rats that were subjected to maternal separation
Por:
Prieto, NMR, Lopez, AR, Morales, MP, Pech, O, Mendez-Diaz, M, Contreras, AER, Prospero-Garcia, O
Publicada:
1 dic 2012
Resumen:
Maternal separation (MS) induces a series of changes in rats' behavior; among them a reduction in spontaneous sleep. One potentially impaired system is the endocannabinoid system (eCBs), since it contributes to generate sleep. To investigate if there are situations early in life that affect the eCBs, which would contribute to make rats vulnerable to suffering insomnia, we studied the rodent model of MS. Rats were separated from their mothers for 3 h-periods daily, from postnatal day (PND) 2 to PND 16. Once they gained 250 g of body weight (adult rats), they were implanted with electrodes to record the sleep-waking cycle (SWC). MS rats and non-MS (NMS) siblings were assigned to one of the following groups: vehicle, oleamide (OLE, an agonist of the cannabinoid receptor 1, CB1R), OLE + AM251 (an antagonist of the CB1R) and AM251 alone. Expression of the CBR1 receptor was also analyzed in the frontal cortex (FCx) and in the hippocampus (HIP) of both NMS and MS rats. Results indicated that
Filiaciones:
Prieto, NMR:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
Lopez, AR:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
Morales, MP:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
Pech, O:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
Mendez-Diaz, M:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
Contreras, AER:
Univ Nacl Autonoma Mexico, Dept Psicofisiol, Fac Psicol, Lab Neurogenom Cognitiva, Mexico City 04510, DF, Mexico
Prospero-Garcia, O:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Grp Neurociencias,Lab Canabinoides, Mexico City 04510, DF, Mexico
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