Sialyl Lewis x expression in cervical scrapes of premalignant lesions


Por: Velázquez-Márquez N., Santos-López G., Jiménez-Aranda L., Reyes-Leyva J., Vallejo-Ruiz V.

Publicada: 1 dic 2012
Resumen:
Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumour progression and metastases. Altered expression of glycosidic antigens has been reported in cervical cancer. In cervix premalignant lesions, an increased expression of sialic acid has been reported. In the present study we determined the expression profiles of the glycosidic antigens Tn, sialyl Tn (sTn), Lewis a (Le(a)), sialyl Lewis a (sLe(a)), Lewis x (Le(x)) and sialyl Lewis x (sLe(x)) in cervical scrapes with cytological diagnoses of normal, low-grade squamous intraepithelial lesions (LGSIL) and high-grade squamous intraepithelial lesions (HGSIL). Cervical scrapings were collected to detect tumour antigens expressions by flow cytometry using monoclonal antibodies. Cytometry analysis of Tn, sTn, Le(a) and Le(x) did not reveal differences at the expression level among groups. The number of positive cells to sLe(a) antigen increased in the HGSIL group with respect to the normal group (p=0.0495).

Filiaciones:
Velázquez-Márquez N.:
 Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Carretera Federal Atlixco-Metepec, Atlixco, Puebla, Mexico C.P. 74360, Mexico

Santos-López G.:
 Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Carretera Federal Atlixco-Metepec, Atlixco, Puebla, Mexico C.P. 74360, Mexico

Jiménez-Aranda L.:
 Clínica de Displasias, Hospital General de Zona No. 1, Instituto Mexicano del Seguro Social, Tlaxcala, Tlaxcala, Mexico, Mexico

Reyes-Leyva J.:
 Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Carretera Federal Atlixco-Metepec, Atlixco, Puebla, Mexico C.P. 74360, Mexico

Vallejo-Ruiz V.:
 Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Carretera Federal Atlixco-Metepec, Atlixco, Puebla, Mexico C.P. 74360, Mexico
ISSN: 02505991
Editorial
INDIAN ACAD SCIENCES, C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA, India
Tipo de documento: Article
Volumen: 37 Número: 6
Páginas: 999-1004
WOS Id: 000312046000011
ID de PubMed: 23151790