Regioselective glucosylation of inositols catalyzed by Thermoanaerobacter sp CGTase
Por:
Miranda-Molina A., Marquina-Bahena S., López-Munguía A., Álvarez L., Castillo E.
Publicada:
1 oct 2012
Resumen:
Monoglucosylated products of L-chiro-, D-chiro-, muco-, and allo-inositol were synthesized by regioselective alpha-D-glucosylation with cyclodextrin glucosyl transferase from Thermoanaerobacter sp. after hydrolysis of by products with Aspergillus niger glucoamylase. While the reactions carried out with D-chiro-, muco-, and allo-inositol resulted in the regioselective formation of monoglucosylated products, two products were obtained in the reaction with L-chiro-inositol. Through the structural characterization of the glucosylated inositols here we demonstrated that the selectivity observed in the glucosylation of several inositols by Thermoanaerobacter sp. CGTase, is analogous to the specificity observed for the glucosylation of beta-D-glucopyranose and equivalent glucosides. (C) 2012 Elsevier Ltd. All rights reserved.
Filiaciones:
Miranda-Molina A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62250, Morelos, Mexico
Marquina-Bahena S.:
Centro de Investigaciones Químicas, Universidad Autónoma Del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca, Morelos 62210, Mexico
López-Munguía A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62250, Morelos, Mexico
Álvarez L.:
Centro de Investigaciones Químicas, Universidad Autónoma Del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca, Morelos 62210, Mexico
Castillo E.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62250, Morelos, Mexico
|