A Spectrum of Pleiotropic Consequences in Development Due to Changes in a Regulatory Pathway
Por:
Escalante A.E., Inouye S., Travisano M.
Publicada:
24 ago 2012
Resumen:
Regulatory evolution has frequently been proposed as the primary mechanism driving morphological evolution. This is because regulatory changes may be less likely to cause deleterious pleiotropic effects than changes in protein structure, and consequently have a higher likelihood to be beneficial. We examined the potential for mutations in trans acting regulatory elements to drive phenotypic change, and the predictability of such change. We approach these questions by the study of the phenotypic scope and size of controlled alteration in the developmental network of the bacterium Myxococcus xanthus. We perturbed the expression of a key regulatory gene (fruA) by constructing independent in-frame deletions of four trans acting regulatory loci that modify its expression. While mutants retained developmental capability, the deletions caused changes in the expression of fruA and a dramatic shortening of time required for completion of development. We found phenotypic changes in the majority
Filiaciones:
Escalante A.E.:
Univ Nacl Autonoma Mexico, Dept Ecol Biodiversidad, Inst Ecol, Mexico City 04510, DF, Mexico
Inouye S.:
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, United States
Travisano M.:
Department of Ecology, Evolution and Behavior, University of Minnesota, St. Paul, MN, United States
Biotechnology Institute, University of Minnesota, St. Paul, MN, United States
Gold
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