Progesterone receptor and SRC-1 participate in the regulation of VEGF, EGFR and Cyclin D1 expression in human astrocytoma cell lines
Por:
Hernandez-Hernandez, OT, Gonzalez-Garcia, TK, Camacho-Arroyo, I
Publicada:
1 oct 2012
Resumen:
Astrocytomas are the most common primary brain tumors in humans. It has been reported that vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), cyclin D1 and progesterone receptor (PR) expression levels are elevated in patients with high-grade astrocytomas. Progesterone (P) regulates astrocytomas growth through its interaction with PR, which recruits coregulatory proteins such as steroid receptor coactivator-1 (SRC-1) that are required for efficient transcriptional activation. The regulation of VEGF, EGFR and cyclin D1 expression by Pin human astrocytoma cells is not known. We studied the role of PR and SRC-1 in the expression of VEGF, EGFR and cyclin D1 mediated by P in human astrocytoma cell lines grade III (U373) and IV (D54). P significantly increased VEGF and EGFR mRNA expression after 12 h of treatment in D54 cells that was reflected at protein level 24 h after treatment. This effect was blocked by the PR antagonist, RU 486. In U373 cells cyclin D1
Filiaciones:
Hernandez-Hernandez, OT:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Coyoacan 04510, Df Mexico, Mexico
Gonzalez-Garcia, TK:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Coyoacan 04510, Df Mexico, Mexico
Camacho-Arroyo, I:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Coyoacan 04510, Df Mexico, Mexico
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