Comparative Proteomic Analysis of Aedes aegypti Larval Midgut after Intoxication with Cry11Aa Toxin from Bacillus thuringiensis
Por:
Cancino-Rodezno A., Lozano L., Oppert C., Castro J.I., Lanz-Mendoza H., Encarnación S., Evans A.E., Gill S.S., Soberón M., Jurat-Fuentes J.L., Bravo A.
Publicada:
16 may 2012
Resumen:
Cry toxins produced by Bacillus thuringiensis bacteria are environmentally safe alternatives to control insect pests. They are pore-forming toxins that specifically affect cell permeability and cellular integrity of insect-midgut cells. In this work we analyzed the defensive response of Aedes aegypti larva to Cry11Aa toxin intoxication by proteomic and functional genomic analyses. Two dimensional differential in-gel electrophoresis (2D-DIGE) was utilized to analyze proteomic differences among A. aegypti larvae intoxicated with different doses of Cry11Aa toxin compared to a buffer treatment. Spots with significant differential expression (p<0.05) were then identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), revealing 18 up-regulated and seven down-regulated proteins. The most abundant subcategories of differentially expressed proteins were proteins involved in protein turnover and folding, energy production, and cytoskeleton maintenance. We selected three candidate
Filiaciones:
Cancino-Rodezno A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62191, Morelos, Mexico
Lozano L.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Oppert C.:
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, United States
Castro J.I.:
Unidad de Proteómica, Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico
Lanz-Mendoza H.:
Unidad de Proteómica, Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico
Encarnación S.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Evans A.E.:
Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, CA, United States
Gill S.S.:
Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, CA, United States
Soberón M.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62191, Morelos, Mexico
Jurat-Fuentes J.L.:
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, United States
Bravo A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62191, Morelos, Mexico
Gold
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