The Srb1+1050T Allele Is Associated with Metabolic Syndrome in Children but Not with Cholesteryl Ester Plasma Concentrations of High-Density Lipoprotein Subclasses
Por:
Juárez-Meavepeña M., Carreón-Torres E., López-Osorio C., García-Sánchez C., Gamboa R., Torres-Tamayo M., Fragoso J.M., Rodríguez-Pérez J.M., Vargas-Alarcón G., Pérez-Méndez O.
Publicada:
1 abr 2012
Resumen:
Background: Low cholesterol and phospholipid plasma levels of some high-density lipoprotein (HDL) subclasses have been described in children with metabolic syndrome. Scavenger receptor class B type I (SR-BI) has been proposed to be at the origin of such HDL alterations because of its key role on cholesteryl esters-HDL metabolism. However, the possible contribution of SR-BI has not been specifically explored in this kind of patients. Methods: Plasma lipid concentrations of HDL subclasses, i.e., triglycerides (TG), phosphatidylcholine (Ph), free cholesterol (FC), and total cholesterol (TC), were determined by enzymatic staining on polyacrylamide gradient gels (PAGE) in 39 pediatric patients with metabolic syndrome and 65 children as controls. Cholesteryl esters were estimated by the difference between TC and FC. Proteins of HDL subclasses were also stained for the assessment of the relative size distribution of HDL. For statistical analysis, the study population was grouped by Srb1 + 105
Filiaciones:
Juárez-Meavepeña M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Carreón-Torres E.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
López-Osorio C.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
García-Sánchez C.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Gamboa R.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Torres-Tamayo M.:
Obesity Clinic, General Hospital Manuel Gea González, Mexico City, Mexico
Fragoso J.M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Rodríguez-Pérez J.M.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Vargas-Alarcón G.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
Pérez-Méndez O.:
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Sección XVI, Juan Badiano 1, 14080 Mexico D.F., Mexico
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