Low molecular weight compounds from Zoanthus sociatus impair insulin secretion via Ca+2 influx blockade and cause glucose intolerance in vivo
Por:
Diaz-Garcia C.M., Sanchez-Soto C., Fuentes-Silva D., Leon-Pinzon C., Dominguez-Perez D., Varela C., Rodriguez-Romero A., Castaneda, O, Hiriart M.
Publicada:
1 feb 2012
Categoría:
Toxicology
Resumen:
Cnidarians comprise a taxon with a high biodiversity of cytolitic, neurotoxic and cardiotoxic compounds, which have not been studied on insulin release. We tested the effect of a crude extract of Zoanthus sociatus (Ellis, 1767) and the low molecular weight fraction of this extract on insulin secretion in isolated rat beta-cells and also in a glucose tolerance test in vivo. We observed that the extract inhibited insulin release by reducing the amount secreted by individual beta-cells and also by silencing a fraction of the secreting population. This effect coincided with a diminished rise of intracellular Ca+2 in response to high glucose and high K+-induced depolarization. Moreover intraperitoneal administration of the low molecular weight fraction produced glucose intolerance in adult rats. The active fraction exhibited molecular weights similar to the neurotoxins described in the phylum. Our results broaden the toxic effects of cnidarian venoms and show evidence of potential modulator
Filiaciones:
Diaz-Garcia C.M.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Div Neurociencias, Dept Neurodesarrollo & Fisiol, Mexico City 04510, DF, Mexico
Sanchez-Soto C.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Div Neurociencias, Dept Neurodesarrollo & Fisiol, Mexico City 04510, DF, Mexico
Fuentes-Silva D.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Biomacromol, Mexico City 04510, DF, Mexico
Leon-Pinzon C.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Div Neurociencias, Dept Neurodesarrollo & Fisiol, Mexico City 04510, DF, Mexico
Dominguez-Perez D.:
Departamento de Biología, Universidad Central Martha Abreu de Las Villas, Carretera a Camajuaní Km 51/2, Santa Clara, CP 50100, Cuba
Varela C.:
Acuario Nacional de Cuba, Avenida 3ra, Esquina 62, Miramar Playa, Ciudad de La Habana, CP 10400, Cuba
Rodriguez-Romero A.:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Biomacromol, Mexico City 04510, DF, Mexico
Hiriart M.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Div Neurociencias, Dept Neurodesarrollo & Fisiol, Mexico City 04510, DF, Mexico
|