An experimental mixed bacterial infection induced differential secretion of proinflammatory cytokines (IL-1 beta, TNF alpha) and proMMP-9 in human fetal membranes
Por:
Flores-Herrera H., García-López G., Díaz N.F., Molina-Hernández A., Osorio-Caballero M., Soriano-Becerril D., Zaga-Clavellina V.
Publicada:
1 abr 2012
Resumen:
Overall, 1-4% of all births in the US are complicated by choriamnionitis. Choriamnionitis is a polymicrobial infection most often due to ascending genital microbes which, in over 65% of positive amniotic fluid cultures, involves two or more organisms. In this study, we determine the cytokines expression (IL-1 beta, TNF alpha) and prometalloproteinase activation (proMMP-2 and proMMP-9) after double o single infection an in vitro model of human fetal membranes. Fetal membranes at term were mounted in the Transwell culture system and after 24 h of infection, choriodecidual, and amnion media was collected. IL-1 beta and TNF alpha were evaluated by ELISA, whereas proMMP-9 and proMMP-2 were determined by substrate gel zymography. The chorioclecidual and amnion compartments actively respond to the infectious process, which induced the secretion of IL-1 beta, TNF alpha, and proMMP-9 after either mixed or single infection. The proMMP-2 secretion profile was the same after all experimental condi
Filiaciones:
Flores-Herrera H.:
Department of Biochemistry and Molecular Biology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Montes Urales # 800, Col. Lomas de Virreyes cp 11000, Mexico City, Mexico
García-López G.:
Department of Cellular Biology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
Díaz N.F.:
Department of Cellular Biology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
Molina-Hernández A.:
Department of Cellular Biology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
Osorio-Caballero M.:
Department of Obstetrics and Gynecology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
Soriano-Becerril D.:
Department of Infectology and Immunology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
Zaga-Clavellina V.:
Department of Cellular Biology, National Institute of Perinatology Isidro Espinosa de Los Reyes, Mexico
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