Translation initiation factor eIF-5A; the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis
Por:
Carvajal-Gamez B.I., Quintas-Granados L.I., Arroyo R., Mendoza-Hernández G., Alvarez-Sánchez M.E.
Publicada:
1 mar 2012
Resumen:
The eukaryotic translation factor elF-5A is highly conserved throughout eukaryotes and undergoes an unusual polyamine-dependent post-translational modification called hypusination. Trichomonas vagina/is has two tveif-5a genes (tveif-5a1 and tveif-5a2), each encoding a 19-kDa protein. In this report, we describe the detection of two forms with different isoelectric points (5.2 and 5.5) that correspond to the precursor and mature TvelF-5A, respectively. In addition, we demonstrated that only the mature form of TvelF-5A is phosphorylated and glycosylated via two-dimensional gel electrophoresis-western blot (2DE-WB) assays using anti-phosphoserine and anti-phosphotyrosine antibodies and the SNA, ConA and MM lectins. Interestingly, when the protozoa were grown in 1,4-diamino-2-butanone (DAB), an inhibitor of putrescine biosynthesis, and transferred to medium containing exogenous putrescine, a new spot with an isoelectric point of 5.3 was observed, presumably corresponding to a phosphorylate
Filiaciones:
Carvajal-Gamez B.I.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100, Mexico City, Mexico
Quintas-Granados L.I.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100, Mexico City, Mexico
Arroyo R.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Av IPN 2508, Col. San Pedro Zacatenco, CP 07360, Mexico City, Mexico
Mendoza-Hernández G.:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico
Alvarez-Sánchez M.E.:
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100, Mexico City, Mexico
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