An Isaria fumosorosea SCAR marker for evaluation of soil, insect, and airborne samples
Por:
Ayala-Zermeno, MA, Reyes-Montes, MR, Arroyo-Vazquez, E, Calderon-Ezquerro, MC, Mier, T, Robledo-Retana, T, Toriello, C
Publicada:
1 ene 2011
Resumen:
Molecular detection markers are needed for ecological studies of entomopathogenic fungi. In this study, a sequence-characterized amplified region (SCAR) marker of Isaria fumosorosea was used to detect the fungus in soil, insects, and airborne samples. These were artificially added with different fungal conidial concentrations. Specificity and sensitivity were tested with semi-nested PCR using oligonucleotides E-AA/M-CTA(124) F and E-AA/M-CTA(124) R for the first amplification and E-AA/M-CTA(124) F and E-AA/M-CTA(103) R for the second amplification. Specificity assays showed a specific band of 103 bp for DNA samples from 10 I. fumosorosea strains used. Negative results were observed for DNA samples from other species of Isaria, including I. amoene-rosea, I. farinosa, and Paecilomyces carneus as well as with other entomopathogens such as Metarhizium acridum, M. anisopliae, M. majus, M. flavoviride Type E, and Lecanicillium lecanii. Sensitivity assays showed that the specific SCAR marker
Filiaciones:
Ayala-Zermeno, MA:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Reyes-Montes, MR:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Arroyo-Vazquez, E:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Calderon-Ezquerro, MC:
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City, DF, Mexico
Robledo-Retana, T:
Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City, DF, Mexico
Toriello, C:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
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