A simple LC-MS/MS method to determine plasma and cerebrospinal fluid levels of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in patients with neurocyst
Por:
González-Hernández I., Ruiz-Olmedo M.I., Cárdenas G., Jung-Cook H.
Publicada:
1 feb 2012
Resumen:
The development and validation of an LC-MS/MS method for the simultaneous determination of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in human plasma are described. Samples of 200 mu L were extracted with etherdichloromethanechloroform (60:30:10, v/v/v). The chromatographic separation was performed using a C(18) column with methanolformic acid 20?mmol/L (70:30) as the mobile phase. The method was linear in a range of 20-5000 ng/mL for albendazole sulfoxide and 10-1500 ng/mL for albendazole sulfone. For both analytes the method was precise (RSD < 12%) and accurate (RE <7%) with high recovery (>90%). The method was successfully applied to determine the plasma and cerebrospinal fluid levels of albendazole sulfoxide and albendazole sulfone in patients with subarachnoidal neurocysticercosis who received albendazole at 30 mg/kg per day for 7 days. This LC-MS/MS method yielded a quick, simple and reliable protocol for determining albendazole sulfoxide and albendaz
Filiaciones:
González-Hernández I.:
Laboratorio de Neuropsicofarmacología, Instituto Nacional de Neurología y Neurocirugía, México, D.F., 14269, Mexico
Ruiz-Olmedo M.I.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Farm, Mexico City 04510, DF, Mexico
Cárdenas G.:
Laboratorio de Infectologia, Instituto Nacional de Neurologia y Neurocirugia, Mexico D.F. 14269, Mexico
Jung-Cook H.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Farm, Mexico City 04510, DF, Mexico
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