Comparative proteome analysis of Brucella abortus 2308 and its virB type IV secretion system mutant reveals new T4SS-related candidate proteins


Por: Paredes-Cervantes V., Flores-Mejía R., Moreno-Lafont M.C., Lanz-Mendoza H., Tello-López Á.T., Castillo-Vera J., Pando-Robles V., Hurtado-Sil G., González-González E., Rodríguez-Cortés O., Gutiérrez-Hoya A., Vega-Ramírez M.T., López-Santiago R.

Publicada: 18 nov 2011
Resumen:
Brucella abortus is an alpha-2 proteobacteria with a type IV secretion system (T4SS) known as virB, which is necessary to gain virulence by building up a replicative vacuole associated with the endoplasmic reticulum of the host cell. A virB T4SS mutant of the B. abortus 2308 strain and its wild-type strain were grown in acid medium in order to obtain and analyze their proteomes, looking for putative proteins that may serve as T4SS substrates and those that may be subjected to T4SS regulation. A total of 47 overexpressed and 22 under-expressed proteins from the virB T4SS mutant strain were selected and sequenced. Some of the 69 analyzed proteins have not been described before either as over or under-expressed in relation to a virB T4SS mutation, whereas some of them have been already described by other groups as potentially important secretory proteins in other Brucella species. An important number of the proteins identified are outer membrane and periplasmic space protein, which makes

Filiaciones:
Paredes-Cervantes V.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

 Centro Médico Nacional La Raza, IMSS, México DF, Mexico

Flores-Mejía R.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Moreno-Lafont M.C.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Lanz-Mendoza H.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Tello-López Á.T.:
 Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico

Castillo-Vera J.:
 Centro Médico Nacional La Raza, IMSS, México DF, Mexico

 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Pando-Robles V.:
 Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico

Hurtado-Sil G.:
 Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico

González-González E.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Rodríguez-Cortés O.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Gutiérrez-Hoya A.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

Vega-Ramírez M.T.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico

López-Santiago R.:
 Departamentos de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico
ISSN: 18743919
Editorial
ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, Países Bajos
Tipo de documento: Article
Volumen: 74 Número: 12
Páginas: 2959-2971
WOS Id: 000298305900033
ID de PubMed: 21875698