MIF Synergizes with Trypanosoma cruzi Antigens to Promote Efficient Dendritic Cell Maturation and IL-12 Production via p38 MAPK
Por:
Terrazas, CA, Huitron, E, Vazquez, A, Juarez, I, Camacho, GM, Calleja, EA, Rodriguez-Sosa, M
Publicada:
1 ene 2011
Resumen:
Macrophage migration inhibitory factor (MIF) has been found to be involved in host resistance to several parasitic infections. To determine the mechanisms of the MIF-dependent responses to Trypanosoma cruzi, we investigated host resistance in MIF-/- mice (on the BALB/c background) during an intraperitoneal infection. We focused on the potential involvement of MIF in dendritic cell (DC) maturation and cytokine production. Following a challenge with 5 x 10(3) T. cruzi parasites, wild type (WT) mice developed a strong IL-12 response and adequate maturation of the draining mesenteric lymph node DCs and were resistant to infection. In contrast, similarly infected MIF(-/-) mice mounted a weak IL-12 response, displayed immature DCs in the early phases of infection and rapidly succumbed to T. cruzi infection. The lack of maturation and IL-12 production by the DCs in response to total T. cruzi antigen (TcAg) was confirmed by in vitro studies. These effects were reversed following treatment with
Filiaciones:
Terrazas, CA:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, Estado De Mexic, Mexico
Huitron, E:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, Estado De Mexic, Mexico
Vazquez, A:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, Estado De Mexic, Mexico
Juarez, I:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, Estado De Mexic, Mexico
Rodriguez-Sosa, M:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, Estado De Mexic, Mexico
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