Differential Expression of Estrogen Receptors in Two Hippocampal Regions During the Estrous Cycle of the Rat
Por:
Mendoza-Garcés L., Mendoza-Rodríguez C.A., Jiménez-Trejo F., Picazo O., Rodríguez M.C., Cerbón M.
Publicada:
1 nov 2011
Resumen:
In the hippocampus, estrogens increase dendritic arborization, long-term potentiation, neuroprotection, and participate in many functions related with learning, memory, and affective behaviors. The presence of both estrogen receptors alpha (ER alpha) and beta (ER beta) isoforms has been described in the hippocampus where they play different physiological roles. The aim of this study was to investigate, by using both techniques immunohistochemistry and Western Blot, the expression pattern of ER alpha and ER beta in the hippocampus of the rat along the estrous cycle. Western blot analysis was used to confirm the specificity of the antibodies used against ER alpha and ER beta and its relative content in the hippocampus. Results from immunohistochemical studies indicate that ER beta expression increased more than the ER alpha in CA1 and CA3 regions during all phases of the estrous cycle. ER beta immunoreactivity was mainly located in the nucleus and predominantly expressed in CA1 during es
Filiaciones:
Mendoza-Garcés L.:
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, Distrito Federal, Mexico
Mendoza-Rodríguez C.A.:
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, Distrito Federal, Mexico
Jiménez-Trejo F.:
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, Distrito Federal, Mexico
Picazo O.:
Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Diaz Miron Col. Sto. Tomás, México, Distrito Federal, Mexico
Rodríguez M.C.:
Centro de Investigación en Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Mexico
Cerbón M.:
Univ Nacl Autonoma Mexico, Biol Lab, Fac Quim, Mexico City 04510, DF, Mexico
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, Distrito Federal, Mexico
|