Autocrine/paracrine erythropoietin signalling promotes JAK/STAT-dependent proliferation of human cervical cancer cells
Por:
Lopez T.V., Lappin, TRJ, Maxwell P., Shi Z., Lopez-Marure R., Aguilar C., Rocha-Zavaleta L.
Publicada:
1 dic 2011
Resumen:
Erythropoietin (Epo) regulates erythropoiesis by binding to its receptor (EpoR) and promoting cell proliferation, differentiation and inhibition of apoptosis. Epo is widely used to treat cervical cancer-related anaemia. However, there are data suggesting that administration of Epo is associated with an increment in recurrence rate, and decreased disease-free and overall survival. In the present study, we investigated the expression of Epo and EpoR on cervical cancer cell lines. We observed that both EpoR and extracellular Epo are constitutively expressed in cervical cancer cells. Inhibition of either Epo or EpoR expression with siRNA attenuated cell proliferation, whereas addition of exogenous Epo led to a significant increase in cell growth, both in vitro and in vivo. Epo-induced proliferation was associated with the activation of JAK2, JAK3, STAT3 and STAT5 but not JAK1 and STAT1. Our results are consistent with the existence of a functional, endogenous Epo/EpoR system in cervical ca
Filiaciones:
Lopez T.V.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Mexico City 04510, DF, Mexico
Maxwell P.:
Centre for Cancer Research and Cell Biology, Queen's University Belfast, United Kingdom
Shi Z.:
Centre for Cancer Research and Cell Biology, Queen's University Belfast, United Kingdom
School of Life Sciences, Kingston University, London, United Kingdom
Lopez-Marure R.:
Instituto Nacional de Cardiologia Ignacio Chavez, Departamento de Biología Celular, Mexico City, Mexico
Aguilar C.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Mexico City 04510, DF, Mexico
Rocha-Zavaleta L.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Mol Biol & Biotechnol, Mexico City 04510, DF, Mexico
Bronze
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