The replication origin of a repABC plasmid
Por:
Cervantes-Rivera, R, Pedraza-Lopez, F, Perez-Segura, G, Cevallos, MA
Publicada:
30 jun 2011
Resumen:
Background: repABC operons are present on large, low copy-number plasmids and on some secondary chromosomes in at least 19 alpha-proteobacterial genera, and are responsible for the replication and segregation properties of these replicons. These operons consist, with some variations, of three genes: repA, repB, and repC. RepA and RepB are involved in plasmid partitioning and in the negative regulation of their own transcription, and RepC is the limiting factor for replication. An antisense RNA encoded between the repB-repC genes modulates repC expression. Results: To identify the minimal region of the Rhizobium etli p42d plasmid that is capable of autonomous replication, we amplified different regions of the repABC operon using PCR and cloned the regions into a suicide vector. The resulting vectors were then introduced into R. etli strains that did or did not contain p42d. The minimal replicon consisted of a repC open reading frame under the control of a constitutive promoter with a Sh
Filiaciones:
Cervantes-Rivera, R:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Pedraza-Lopez, F:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Perez-Segura, G:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
Cevallos, MA:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62191, Morelos, Mexico
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