The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain
Por:
Cervantes, L, Bustos, P, Girard, L, Santamaria, RI, Davila, G, Vinuesa, P, Romero, D, Brom, S
Publicada:
25 jun 2011
Resumen:
Background: Bean-nodulating Rhizobium etli originated in Mesoamerica, while soybean-nodulating Sinorhizobium fredii evolved in East Asia. S. fredii strains, such as GR64, have been isolated from bean nodules in Spain, suggesting the occurrence of conjugative transfer events between introduced and native strains. In R. etli CFN42, transfer of the symbiotic plasmid (pRet42d) requires cointegration with the endogenous self-transmissible plasmid pRet42a. Aiming at further understanding the generation of diversity among bean nodulating strains, we analyzed the plasmids of S. fredii GR64: pSfr64a and pSfr64b (symbiotic plasmid). Results: The conjugative transfer of the plasmids of strain GR64 was analyzed. Plasmid pSfr64a was self-transmissible, and required for transfer of the symbiotic plasmid. We sequenced pSfr64a, finding 166 ORFs. pSfr64a showed three large segments of different evolutionary origins; the first one presented 38 ORFs that were highly similar to genes located on the chromo
Filiaciones:
Cervantes, L:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Ingn Genom, Cuernavaca 62240, Morelos, Mexico
Bustos, P:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62240, Morelos, Mexico
Girard, L:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Cuernavaca 62240, Morelos, Mexico
Santamaria, RI:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62240, Morelos, Mexico
Davila, G:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Evolut, Cuernavaca 62240, Morelos, Mexico
Vinuesa, P:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Ingn Genom, Cuernavaca 62240, Morelos, Mexico
Romero, D:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Ingn Genom, Cuernavaca 62240, Morelos, Mexico
Brom, S:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Ingn Genom, Cuernavaca 62240, Morelos, Mexico
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