Keloid skin scars: The influence of hyperbaric oxygenation on fibroblast growth and on the expression of messenger RNA for insulin like growth factor and for transforming growth factor
Por:
Romero-Valdovinos M., Cárdenas-Mejía A., Gutiérrez-Gómez C., Flisser A., Kawa-Karasik S., Ortiz-Monasterio F.
Publicada:
1 ago 2011
Resumen:
Wound healing can result in the development of keloid scars that contain atypical fibroblasts and an overabundance of extracellular matrix components. Hyperbaric oxygenation (HBO) refers to exposure to pure oxygen under increased atmospheric pressure and is recognized as a valuable supplementary method of treatment for problematic wounds. The effect of HBO in the expression of insulin-like growth factor type 1 (ILGF-1) and transforming growth factor ß (TGF-ß) messenger RNAs was determined by semiquantitative RT-PCR in fibroblasts obtained from keloid scars and nonwound involved skin fibroblast from the same patient. ILGF-1 and TGF-ß are the principal mitogens during wound regeneration. We found a decrease in the growth of fibroblasts and in the expression of ILGF-1 and TGF-ß messengers in keloid and nonkeloid fibroblast after chronic exposition to hyperbaric oxygenation compared with normal oxygen partial pressure. © 2011 The Society for In Vitro Biology.
Filiaciones:
Romero-Valdovinos M.:
Departamento de Biología Molecular e Histocompatibilidad, Mexico City, Mexico
Cárdenas-Mejía A.:
División de Cirugía Plástica y Reconstructiva, Hospital General Dr. Manuel Gea González, Mexico City, Mexico
Gutiérrez-Gómez C.:
División de Cirugía Plástica y Reconstructiva, Hospital General Dr. Manuel Gea González, Mexico City, Mexico
Flisser A.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Mexico City, DF, Mexico
Kawa-Karasik S.:
Departamento de Biología Molecular e Histocompatibilidad, Mexico City, Mexico
Ortiz-Monasterio F.:
División de Cirugía Plástica y Reconstructiva, Hospital General Dr. Manuel Gea González, Mexico City, Mexico
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