Aggregation of Human Serum Albumin on Graphite and Single-Walled Carbon Nanotubes as Studied by Scanning Probe Microscopies
Por:
Rodríguez-Galván A., Contreras-Torres F.F., Basiuk E.V., Alvarez-Zauco E., Heredia A., Basiuk V.A.
Publicada:
1 jun 2011
Resumen:
Human serum albumin (HSA) is the most abundant protein in blood plasma showing a remarkable ability to bind a broad range of hydrophobic substrates. We employed scanning tunneling microscopy and atomic force microscopy to characterize the morphology of HSA aggregates on highly-ordered pyrolytic graphite (HOPG) and single-walled carbon nanotubes (SWNTs). The morphologies found for albumin aggregates on HOPG are quite different from the ones observed on SWNTs. On HOPG, HSA forms aggregates of roughly 10-20 molecules; single protein molecules were observed as well. In the case of SWNTs, nanotubes were partially or totally covered with HSA, exhibiting four general types of aggregation: (i) SWNT sidewalls contain single molecules of albumin which are away from each other at distances longer than the HSA molecular size; (ii) SWNTs are completely covered with HSA, which forms a thin and relatively homogeneous layer; (iii) SWNTs have a complete layer of HSA with additional accumulation of prot
Filiaciones:
Rodríguez-Galván A.:
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Mexico City 04510, DF, Mexico
Contreras-Torres F.F.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Aplicadas & Desarrollo Tecnol, Mexico City 04510, DF, Mexico
Basiuk E.V.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Aplicadas & Desarrollo Tecnol, Mexico City 04510, DF, Mexico
Alvarez-Zauco E.:
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Mexico City 04510, DF, Mexico
Heredia A.:
Department of Ceramics and Glass Engineering, Center for Research in Ceramics and Composites Materials, University of Aveiro, 3810-193, Aveiro, Portugal
Basiuk V.A.:
Univ Nacl Autonoma Mexico, Inst Ciencias Nucl, Mexico City 04510, DF, Mexico
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