Consecutive Low Doses of Cyclosporine A Induce Pro-Inflammatory Cytokines and Accelerate Allograft Skin Rejection
Por:
López-Flores R., Bojalil R., Benítez J.C., Ledesma-Soto Y., Terrazas C.A., Rodríguez-Sosa M., Terrazas L.I.
Publicada:
1 may 2011
Resumen:
Cyclosporine A (CsA) is a fungus-derived molecule with potent immunosuppressive activity that has been largely used to downregulate cell-mediated immune responses during transplantation. However, previous data have indicated that CsA shows immunomodulatory activity that relays on the antigen concentration and the dose of CsA used. To test the hypothesis that minimal doses of CsA may show different outcomes on grafts, we used an experimental model for skin transplants in mice. ICR outbred mice received skin allografts and were either treated daily with different doses of CsA or left untreated. Untreated mice showed allograft rejection within 14 days, with graft necrosis, infiltration of neutrophils and macrophages and displayed high percentages of CD8(+) T cells in the spleens, which were associated with high serum levels of IL-12, IFN-gamma and TNF-alpha. As expected, mice treated with therapeutic doses of CsA (15 mg/kg) did not show allograft rejection within the follow-up period of 3
Filiaciones:
López-Flores R.:
Carrera de Medicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. de los Barrios Num 1, Los Reyes Iztacala, Tlalnepantla, Estado de México, Mexico
Bojalil R.:
Departamento de Inmunología, Instituto Nacional de Cardiología, Juan Badiano Num1, Tlalpan, México, Mexico
Benítez J.C.:
Carrera de Medicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. de los Barrios Num 1, Los Reyes Iztacala, Tlalnepantla, Estado de México, Mexico
Ledesma-Soto Y.:
UNAM, Unidad Biomed, Fac Estudios Super Iztacala, Tlalnepantla, Estado Mexic, Mexico
Terrazas C.A.:
UNAM, Unidad Biomed, Fac Estudios Super Iztacala, Tlalnepantla, Estado Mexic, Mexico
Rodríguez-Sosa M.:
UNAM, Unidad Biomed, Fac Estudios Super Iztacala, Tlalnepantla, Estado Mexic, Mexico
Terrazas L.I.:
UNAM, Unidad Biomed, Fac Estudios Super Iztacala, Tlalnepantla, Estado Mexic, Mexico
Gold
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