Analysis of plasma membrane Ca2+-ATPase gene expression during epileptogenesis employing single hippocampal CA1 neurons
Por:
Bravo-Martinez, J, Delgado-Coello, B, Garcia, DE, Mas-Oliva, J
Publicada:
1 abr 2011
Resumen:
Disruption of calcium homeostasis in epileptic cells is characterized by both short- and long-term perturbations of Ca2+ buffering systems. Along with the Na+/Ca2+ exchanger, the plasma membrane Ca2+-ATPase (PMCA) plays an important role in excitable cells. The involvement of PMCAs in epileptogenesis has primarily been studied in brief intervals after various stimuli; however, the specific contribution of this molecule to epileptogenesis is not yet fully understood. Our aim has been to investigate whether PMCA expression in the chronic stages of epilepsy is altered. Through an interdisciplinary approach, involving whole-cell recordings and real-time reverse transcriptase-polymerase chain reaction, we have shown that epileptic neurons in our preparation consistently show changes in electrical properties during the period of chronic epilepsy. These changes included increased spike frequency, altered resting membrane potential and changes in passive membrane properties. Following these ob
Filiaciones:
Bravo-Martinez, J:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Mexico City 04510, DF, Mexico
Delgado-Coello, B:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Garcia, DE:
Univ Nacl Autonoma Mexico, Dept Fisiol, Fac Med, Mexico City 04510, DF, Mexico
Mas-Oliva, J:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
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