TRPM8 in Mouse Sperm Detects Temperature Changes and May Influence the Acrosome Reaction
Por:
Martínez-López P., Trevino, CL, de la Vega-Beltrán J.L., De Blas G., Monroy E., Beltrán C., Orta G., Gibbs G.M., O'Bryan M.K., Darszon A.
Publicada:
1 jun 2011
Resumen:
Changes in the concentration of intracellular Ca(2+) ([Ca(2+)](i)) trigger and/or regulate principal sperm functions during fertilization, such as motility, capacitation, and the acrosome reaction (AR). Members of the large TRP channel family participate in a variety of Ca(2+)-dependent cell signaling processes. The eight TRPM channel members constitute one of the seven groups belonging to this family. Here we document using RT-PCR experiments the presence of Trpm2, 4, 7, and 8 in mouse spermatogenic cells. Trpm8 transcription is up-regulated after day 30. The localization of TRPM8 protein in mouse sperm was confirmed by immunocytochemistry and Western blots. Patch clamp recordings in testicular mouse sperm revealed TRPM8 agonist (menthol and icilin) activated currents sensitive to TRPM8 inhibitors N-(4-t-Butylphenyl)-4-(3-Chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carboxamide (BCTC) and capsazepine. These findings are consistent with the presence of functional TRPM8 in mouse sperm. F
Filiaciones:
Martínez-López P.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Trevino, CL:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
de la Vega-Beltrán J.L.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
De Blas G.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Monroy E.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Beltrán C.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Orta G.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
Gibbs G.M.:
Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia
O'Bryan M.K.:
Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia
The Australian Research Council Centre of Excellence in Biotechnology and Development, Monash University, Melbourne, Australia
Darszon A.:
UNAM, Dept Genet Desarrollo & Fisiol Mol, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
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