The Role of Calcium and Nitric Oxide During Liver Enzyme Release Induced by Increased Physical Forces as Evidenced in Partially Hepatectomized Rats
Por:
Díaz-Juárez J., Hernández-Muñoz R.
Publicada:
1 mar 2011
Resumen:
Although increased plasma enzyme activities could be diagnostic for tissue damage, the mechanisms controlling cellular enzyme release remain poorly understood. We found a selective and drastic elevation of serum enzyme activities accompanying rat liver regeneration after partial hepatectomy (PH), apparently controlled by a mechanism dependent on flow-bearing physical forces. In fact, this study assesses a putative role of calcium mobilization and nitric oxide (NO) production underlying rat liver enzyme release. The role of increased shear stress (by enhancing viscosity during perfusion) and the participation of cell calcium and NO were tested in isolated livers subjected to increasing flow rate. After PH, there was a drastic elevation of serum activities for liver enzyme markers, clearly predominating those of mitochondrial localization. Liver enzyme release largely depended on extracellular calcium entry, probably mediated by stretch-sensitive calcium channels, as well as by increasin
Filiaciones:
Díaz-Juárez J.:
Department of Pharmacology, Instituto Nacional Cardiología Ignacio Chávez, Mexico City, Distrito Federal, Mexico
Hernández-Muñoz R.:
Univ Nacl Autonoma Mexico, Inst Cellular Physiol, Dept Cellular Biol, Mexico City 04510, DF, Mexico
Bronze
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